Rabbit Anti-CCR-1/CD191 antibody

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CC chemokine receptor 1; A630091E18Rik; CC-CKR-11; CCBP2; CCR10; CCR11; CCRL1; CCX-CKR; CCX-CKR1; CKR-11; Cmkbrl1; LOC684092; LOC685243; PPR1; VSHK1; C C chemokine receptor type 1; C C CKR 1; CC CKR 1; CCR 1; CD 191; CD191; CD191; Chemokine (C-C motif) re

Cat:

SL1169R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human CCR-1:301-355/355

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: lymph (Mouse) Lysate at 40 ugPrimary: Anti-CCR-1 (SL1169R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 46 kDSample: Bones (Mouse) Lysate at 40 ugPrimary: Anti- CCR-1 (SL1169R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDObserved band size: 45 kDTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CCR1 Polyclonal Antibody, Unconjugated(SL1169R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq, Jul 2008]

Function:
Receptor for a SLCC type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

Subunit:
Interacts with CREB3.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed in different hematopoietic cells.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P32246

Gene ID:
1230

Database links:

Entrez Gene: 1230 Human

Entrez Gene: 12768 Mouse

Omim: 601159 Human

SwissProt: P32246 Human

SwissProt: P51675 Mouse

Unigene: 301921 Human

Unigene: 274927 Mouse

趋化因子是当今细胞因子研究领域的热点之一,它参与多种免疫及炎症反应,在感染、肿瘤的生长与转移、组织修复及创伤愈合等病理生理过程中发挥重要作用.
SLCC型趋化因子受体1(CCR1)主要在嗜酸粒细胞，Th2淋巴细胞，肥大细胞，支气管上皮细胞上表达.CCR1在肿瘤的生长与转移方面起到一定的作用. Picture

Sample: Bones (Mouse) Lysate at 40 ug
Primary: Anti- CCR-1 (SL1169R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 40 kD
Observed band size: 45 kD

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CCR1 Polyclonal Antibody, Unconjugated(SL1169R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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