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Home > Product > Antibody > Rabbit Anti-CD144/VE Cadherin antibody
VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_H
Cat:
SL0878R
Species Reactivity：
Human,Mouse,Rat,
Immunogen：
KLH conjugated synthetic peptide derived from mouse Vascular endothelial cell cadherin:601-700/784<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: HUVEC (Human) Cell Lysate at 30 ug Lane 3: A431 (Human) Cell Lysate at 30 ug Lane 4: LOVO (Human) Cell Lysate at 30 ug Lane 5: SiHa (Human) Cell Lysate at 30 ug Primary: Anti-CD144/VE Cadherin (SL0878R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kDTissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD144/VECD/VE Cadherin Polyclonal Antibody, Unconjugated(SL0878R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingImages provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(SL0878R) 1:100, overnight at 4℃, The secondary antibody was diluted to 1:100 for 120 minutes at 37∑C.Blank control: RSC96 Cells (blue). Primary Antibody: Rabbit Anti- CD144/VE Cadherin/AF488 Conjugated antibody (SL0878R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.ProtocolThe cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (SL0878R-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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Product PDFs
Datasheet:


This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008].

Function:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.

Subunit:
Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1.

Subcellular Location:
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.

Tissue Specificity:
Endothelial tissues and brain.

Post-translational modifications:
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.

Similarity:
Contains 5 cadherin domains.

SWISS:
P55284

Gene ID:
12562

Database links:

Entrez Gene: 1003 Human

Entrez Gene: 12562 Mouse

Entrez Gene: 307618 Rat

Omim: 601120 Human

SwissProt: P33151 Human

SwissProt: P55284 Mouse

Unigene: 76206 Human

Unigene: 21767 Mouse

Unigene: 224644 Rat



血管内皮钙粘蛋白(Vascularendothelial cell cadherin;VE-cadherin;VECD),又名CD144,Cadherin-5,是AJ连接（粘附连接）的主要粘附分子,是维持血管内皮细胞极性和完整性必不可少的内皮细胞特异性钙粘蛋白. Picture

Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: HUVEC (Human) Cell Lysate at 30 ug
Lane 3: A431 (Human) Cell Lysate at 30 ug
Lane 4: LOVO (Human) Cell Lysate at 30 ug
Lane 5: SiHa (Human) Cell Lysate at 30 ug
Primary: Anti-CD144/VE Cadherin (SL0878R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD144/VECD/VE Cadherin Polyclonal Antibody, Unconjugated(SL0878R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(SL0878R) 1:100, overnight at 4℃, The secondary antibody was diluted to 1:100 for 120 minutes at 37∑C.
Blank control: RSC96 Cells (blue).
Primary Antibody: Rabbit Anti- CD144/VE Cadherin/AF488 Conjugated antibody (SL0878R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (SL0878R-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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