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Home > Product > Antibody > Rabbit Anti-CRP antibody
C-reactive protein; C Reactive Protein; C reactive protein pentraxin related; CRP; MGC88244; PTX 1; PTX1.C反应蛋白
Cat:
SL0155R
Species Reactivity：
Human,Mouse,(predicted: Rat,Dog,Pig,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human CRP:125-224/224
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP) Polyclonal Antibody, Unconjugated (SL0155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(SL0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(SL0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


Pentraxins are plasma proteins characterized by their pentameric discoid assembly and their Ca2+ dependent ligand binding, such as Serum amyloid P component (SAP) and SLCreactive Protein (CRP), which are cytokine-inducible acute-phase proteins implicated in innate immunity. CRP binds to ligands containing phosphocholine, SAP binds to amyloid fibrils, DNA, chromatin, fibronectin, C4-binding proteins and glycosaminoglycans. "Long" pentraxins have N-terminal extensions to the common pentraxin domain; one group, the neuronal pentraxins, may be involved in synapse formation and remodeling, and they may also be able to form heteromultimers.

Function:
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Subunit:
Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.

Subcellular Location:
Secreted.

Tissue Specificity:
Found in plasma.

Similarity:
Belongs to the pentaxin family.
Contains 1 pentaxin domain.

SWISS:
P02741

Gene ID:
1401

Database links:

Entrez Gene: 1401 Human

Omim: 123260 Human

SwissProt: P02741 Human

Unigene: 76452 Human



C反应蛋白 CRP（SLCreactive protin）是与C性肺炎球菌的多糖起反应的一种蛋白，在某些细菌的感染过程中出现在血浆中。
CRP是在一些病理性情况下出现于病人血清中的一种糖蛋白，特别是在伴有肯炎症和组织坏死疾病的急性期。血清中出现的这种异常蛋白能和肺炎SLC多糖体发生沉淀反应，当疾病好转或缓解时，CRP减低或消失。
临床意义:
(1)各种急性化脓性炎症、菌血症、组织坏死(心肌梗塞、手术、严重创伤、烧伤等)、恶性肿瘤、结缔组织病、肾移殖后急性排异反应、急性风湿热、风湿性关节炎、类风湿性关节炎、心肌炎急性期、肺炎、肺结核、系统性红斑狼疮、急性肝炎和阑尾炎等，血清中CRP含量可增高，它的出现比其它急性期的反应物质早，所以对疾病的早期诊断很有帮助。
CRP可以鉴别细菌炎症或者是病毒性炎症。细菌性炎症CRP可以明显增高，而病毒性炎症CRP的血清含量一般都在正常范围。 Picture

Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP) Polyclonal Antibody, Unconjugated (SL0155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(SL0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(SL0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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