Rabbit Anti-Estrogen receptor beta antibody

Home > Product > Antibody > Rabbit Anti-Estrogen receptor beta antibody

Estrogen receptor-beta; Estrogen receptor beta; ER BETA; ER-BETA; ER-beta; Erb; ESR 2; ESR 2; ESR B; ESR-B; ESR BETA; ESR-BETA; ESR2; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrog

Cat:

SL0116R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human ER-beta:201-300/530

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Testis (Mouse) Lysate at 40 ugLane 2: Testis (Rat) Lysate at 40 ugLane 3: Ovary (Mouse) Lysate at 40 ugLane 4: Uterus (Mouse) Lysate at 40 ugLane 5: Uterus (Rat) Lysate at 40 ugPrimary: Anti-Estrogen receptor beta (SL0116R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55-59 kDObserved band size: 58-62 kDSample: Kidney(Mouse) lysate at 30ug;Brain(Rat) lysate at 30ug; Primary: Anti-ER-beta (SL0116R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; Predicted band size : 58kDObserved band size : 58kDPositive control: HelaIsotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA.Blank control: A431. Primary Antibody (green line): Rabbit Anti-Estrogen receptor beta antibody (SL0116R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes a member of the family of estrogenreceptors and superfamily of nuclear receptor transcriptionfactors. The gene product contains an N-terminal DNA binding domainand SLCterminal ligand binding domain and is localized to thenucleus, cytoplasm, and mitochondria. Upon binding to17beta-estradiol or related ligands, the encoded protein formshomo- or hetero-dimers that interact with specific DNA sequences toactivate transcription. Some isoforms dominantly inhibit theactivity of other estrogen receptor family members. Severalalternatively spliced transcript variants of this gene have beendescribed, but the full-length nature of some of these variants hasnot been fully characterized. [provided by RefSeq, Jul 2008].

Function:
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Subunit:
Interacts with SLC30A9 (By similarity). Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.

Similarity:
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q92731

Gene ID:
2100

Database links:

Entrez Gene: 2100 Human

Entrez Gene: 13983 Mouse

Entrez Gene: 25149 Rat

Omim: 601663 Human

SwissProt: Q92731 Human

SwissProt: O08537 Mouse

SwissProt: Q62986 Rat

Unigene: 660607 Human

类固醇受体（Steroid Receptors）
Picture

Sample:
Kidney(Mouse) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-ER-beta (SL0116R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000;
Predicted band size : 58kD
Observed band size : 58kD

Positive control: Hela
Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA.

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Estrogen receptor beta antibody (SL0116R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall