Rabbit Anti-IGF 1 antibody | Sunlong Medical

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IBP1; IGF 1; IGF I; IGF-1;IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Full=Insulin-like growth factor I; Insulin Like Growth Factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor

Cat:

SL0014R

Species Reactivity：

Human,Mouse,Rat,Dog,(predicted: Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human IGF-1:49-100/195

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human IGF1 protein (full length) Overexpression E.coli Lysate at 4 ugLane 2: Control (-) E.coli Lysate at 4 ugPrimary: Anti-IGF 1 (SL0014R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 21 kDParaformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Function:
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

Subcellular Location:
Secreted.

DISEASE:
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

Similarity:
Belongs to the insulin family.

SWISS:
P05019

Gene ID:
3479

Database links:

Entrez Gene: 3479 Human

Omim: 14788 Human

SwissProt: P05019 Human

Unigene: 160562 Human

胰岛素样生长因子1(IGF-1)是一种生长调节激素，由肝分泌并入血液循环的中性多肽，具有调节生长和代谢、胰岛素样及促细胞分裂的活性，主要作用于成人。 Picture

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF 1) Polyclonal Antibody, Unconjugated (SL0014R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Specific References (19) | SL0014R has been referenced in 19 publications.

[IF=2.005] Yongfu La. et al. Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak. 2021 Nov 09 WB ; Yak.

PubMed:34752661

[IF=3.335] XuTing Song. et al. Molecular cloning, expression, and functional features of IGF1 splice variants in sheep. Endocr Connect. 2021 Jul;-1(aop): WB ; Sheep.

PubMed:34319906

[IF=5.201] Li Taotao. et al. Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development. Front Cell Dev Biol. 2021 Mar;9:650 IF ; Sheep.

PubMed:33816472

[IF=4.268] Qin Yang. et al. Gypenoside XLIX protects against acute kidney injury by suppressing IGFBP7/IGF1R-mediated programmed cell death and inflammation. Phytomedicine. 2021 Mar;:153541 WB ; Human.

PubMed:33773190

[IF=2.985] Ito E et al. Food restriction reduces cortical bone mass and serum insulin-like growth factor-1 levels and promotes uterine atrophy in miceBiochem Biophys Res Commun.2021 Jan 1;534:165-171. IHC ; Mouse.

PubMed:33288195

[IF=3.598] Li R et al. Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Biofactors. 2019 Oct 24. IHF&ICF ; Human.

PubMed:31647596

[IF=1.812] Liao, Wei-Tao, et al. "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." (2016). IHSLCP ; Rat.

PubMed:29593812

[IF=1.8] Uysal, Nazan, et al. "Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats." Journal of Chemical Neuroanatomy (2017). IHSLCP ; Rat.

PubMed:28179125

[IF=0.48] Gabr, Hala, et al. "The effect of bone marrow‐derived mesenchymal stem cells on chemotherapy induced ovarian failure in albino rats." Microscopy Research and Technique (2016). IHSLCP ; Rat.

PubMed:27453009

[IF=1.422] Muller et al. Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs. (2017) Reprod.Domest.Anim. 52 Suppl 2:187-192 IHC ; Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,.

PubMed:28101891

[IF=3.36] Zhao L et al. Reducing macrophage numbers alleviates temporomandibular joint ankylosis. Cell Tissue Res. 2019 Sep 14. IHC ; Human.

PubMed:31522279

[IF=2.466] Cemile Merve Seymen. et al. Involvement of endometrial IGF-1R/IGF-1/Bcl-2 pathways in experimental polycystic ovary syndrome: Identification of the regulatory effect of melatonin. Tissue Cell. 2021 Jun;:101585 WB,IHC ; Rat.

PubMed:10.1016/j.tice.2021.101585

[IF=1.06] Baykara, B., et al. "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum corticosterone levels in immature rats." Biotechnic & Histochemistry 0 (2013): 1-8. Rat.

PubMed:2396228

[IF=2.08] Yokohira M et al. Lung proliferative lesion‐promoting effects of left pulmonary ligation in A/J female mice. Pathol Int. 2020 Feb 21. IHSLCP ; Mouse.

PubMed:6483387

[IF=2.564] Balogh O et al. Expression of insulin-like growth factor 1 and its receptor in preovulatory follicles and in the corpus luteum in the bitch. Gen Comp Endocrinol. 2018 Dec 1;269:68-74. IHSLCP ; Dog.

PubMed:30125572

[IF=1.97] Liao et al. The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation. (2018) Arch.Med.Sci. 14:370-380 IHC ; Rat.

PubMed:29593812

[IF=1.21] Müller, L., et al. "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." Reproduction in Domestic Animals (2017). IHSLCP ; Dog.

PubMed:10.1111/rda.12896

[IF=1.06] Kiray, M., et al. "Effects of carbon dioxide exposure on early brain development in rats." Biotechnic & Histochemistry 0 (2014): 1-13. IHSLCP ; Rat.

PubMed:24476563