Rabbit Anti-Maspin antibody

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mammary serine proteinase inhibitor; Maspin; Ovalbumin; PI-5; PI5; Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; Serpin B5; serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; SERPINB

Cat:

SL0792R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human Maspin:301-375/375

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Skin Lysates Lane 2: Rat Skin Lysates Primary: Anti-Maspin (SL0792R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42kDaObserved band size: 42kDaSample: MDA-MB-231(Human) Cell Lysate at 40 ugPrimary: Anti-Maspin (SL0792R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kDTissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(SL0792R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(SL0792R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Maspin protein is a sour depressant of nenzyme of a kind of silk ammonia that represses the tumor, is the sour depressant of protei nenzyme one member within super household of the silk ammonia, it is in repress the tumor growth and transfer exertive certain function.

Function:
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Subunit:
Interacts with IRF6.

Subcellular Location:
Secreted, extracellular space.

Tissue Specificity:
Normal mammary epithelial cells.

Similarity:
Belongs to the serpin family. Ov-serpin subfamily.

SWISS:
P36952

Gene ID:
5268

Database links:

Entrez Gene: 5268 Human

Entrez Gene: 20724 Mouse

Entrez Gene: 116589 Rat

Omim: 154790 Human

SwissProt: P36952 Human

SwissProt: P70124 Mouse

SwissProt: P70564 Rat

Unigene: 55279 Human

Unigene: 268618 Mouse

Unigene: 25752 Rat

Maspin蛋白是一种抑制肿瘤的丝氨酸蛋白酶抑制剂, 是丝氨酸蛋白酶抑制剂超家族中的一员，它在抑制肿瘤生长和转移中发挥一定作用。 Picture

Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-Maspin (SL0792R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Tissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(SL0792R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(SL0792R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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