Rabbit Anti-MMP3 antibody | Sunlong Medical

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MMP3_HUMAN; Stromelysin-1; EC:3.4.24.17; STMY1; SL-1; SL 1; SL1; Matrix metalloproteinase-3 (MMP-3); MMP-3; MMP 3; Transin-1;

Cat:

SL0413R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Horse,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human MMP3:401-477/477

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti-MMP-3 (SL0413R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42/52 kDObserved band size: 52 kDSample:Lymph node (Mouse) Lysate at 30 ugPrimary: Anti- MMP-3 (SL0413R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42/52kDObserved band size: 52kDTissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MMP-3 Polyclonal Antibody, Unconjugated(SL0413R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].

Function:
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).

Similarity:
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

SWISS:
P08254

Gene ID:
4314

Database links:

Entrez Gene: 4314 Human

Entrez Gene: 17392 Mouse

Entrez Gene: 171045 Rat

Omim: 185250 Human

SwissProt: P08254 Human

SwissProt: P28862 Mouse

SwissProt: P03957 Rat

Unigene: 375129 Human

合成与降解（Synthesis and Degradation）

基质金属蛋白酶(matrix metalloproteinases, MMPs)是一族依赖锌离子而降解各种细胞外基质的蛋白酶，亦称IV型胶原酶或称明胶酶A，其主要功能为降解IV型胶原，因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。
MMP3目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与肿瘤转移浸润的研究。

Picture

Sample:Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- MMP-3 (SL0413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42/52kD
Observed band size: 52kD

Tissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MMP-3 Polyclonal Antibody, Unconjugated(SL0413R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Specific References (21) | SL0413R has been referenced in 21 publications.

[IF=20.722] Fanqi Hu. et al. Identification of inflammatory regulation roles of thalidomide/ruxolitinib in nucleus pulposus and construction of polyelectrolyte nanocomplexes-impregnated injectable hydrogels for synergistic intervertebral disk degeneration treatment. Nano Today. 2022 Jun;44:101462 WB ; Human.

PubMed:10.1016/j.nantod.2022.101462

[IF=2.586] Cansu Gül Koca. et al. Effect of Single-Dose Locally Applied Lactoferrin on Autograft Healing in Peri-Implant Bone in Rat Models. Injury. 2021 Dec;: IHC ; Rat.

PubMed:35042599

[IF=2.362] Shi, Bohui. et al. Polymorphism of MMP-3 gene and imbalance expression of MMP-3 / TIMP-1 in articular cartilage are associated with an endemic osteochondropathy, Kashin- Beck disease. Bmc Musculoskel Dis. 2022 Dec;23(1):1-7 WB ; Human.

PubMed:34196041

[IF=4.162] Wenwen Chen. et al. Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Des Dev Ther. 2021; 15: 4125–4140 IHC ; mouse.

PubMed:34616145

[IF=7.097] Liting Cheng. et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta Pharm Sin B. 2021 May;: IF ; Mouse.

PubMed:10.1016/j.apsb.2021.04.020

[IF=3.857] Wenqiang Yan. et al. Chondral Defects Cause Kissing Lesions in a Porcine Model:. Cartilage. 0;(): IHC ; Pig.

PubMed:32830514

[IF=2.049] Wang W et al. Ginkgo biloba extract may alleviate viral myocarditis by suppression of S100A4 and MMP‐3. J Med Virol. 2019 Jul 30. IHSLCP ; Mouse.

PubMed:31359441

[IF=2.999] Chen CL et al. miR‐134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP 1 and MMP 3 in vitro and in vivo. FEBS Lett. 2019 Apr 12. WB ; Human.

PubMed:30977909

[IF=1.894] Bei M et al. A Novel Rat Model of Patellofemoral Osteoarthritis Due to Patella Baja, or Low-Lying Patella. Med Sci Monit. 2019 Apr 13;25:2702-2717. IHSLCP ; Rat.

PubMed:30979862

[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat.

PubMed:27665780

[IF=2.47] Luo, Yang, et al. "The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model." European Spine Journal (2014): 1-11. IHSLCP ; Rat.

PubMed:25304649

[IF=3.269] Mengxue Zhang. et al. Identification and validation of potential novel biomarkers for oral squamous cell carcinoma. Bioengineered. 2021;12(1):8845-8862 IF,IHC ; Human,Mouse.

PubMed:3921286

[IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646 WB ; rat.

PubMed:34536814

[IF=4.545] Kai Zhao. et al. Rhizoma drynariae total flavonoids inhibit the inflammatory response and matrix degeneration via MAPK pathway in a rat degenerative cervical intervertebral disc model. Biomed Pharmacother. 2021 Jun;138:111466 IHC ; Rat.

PubMed:33740525

[IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human.

PubMed:31383786

[IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013). IHSLCP ; Rat.

PubMed:23500174

[IF=1.8] Han, Jing, et al. "Long-Term Selenium-Deficient Diet Induces Liver Damage by Altering Hepatocyte Ultrastructure and MMP1/3 and TIMP1/3 Expression in Growing Rats." Biological Trace Element Research (2016): 1-9. IHSLCP ; Rat.

PubMed:27339256

[IF=2.14] Lu, Minling, et al. "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." Cells Tissues Organs 196.3 (2012): 241-250. IHSLCP ; Rabbit.

PubMed:22538829

[IF=1.43] Wang, Xiao-yan, et al. "AMD620 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016). IHSLCP ; Rat.

PubMed:26851314

[IF=2.43] Wei, Fuxin, et al. "Pingyangmycin-induced in vivo lumbar disc degeneration model of rhesus monkeys." Spine 40.4 (2015): E199-E210. IHSLCP ;

PubMed:25679953

[IF=0.98] Koch, Holger, et al. "Tissue engineering of ureteral grafts: Preparation of biocompatible crosslinked ureteral scaffolds of porcine origin." Name: Frontiers in Bioengineering and Biotechnology 3 (2015): 89. IHSLCP ; Pig.

PubMed:26157796