Rabbit Anti-Sodium hydrogen exchanger 1 antibody

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amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+ H+ antiporter amiloride-sensitive; Na+ H+ antiporter; Na+ H+ exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN;

Cat:

SL0505R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human NHE1:470-550/815<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-NHE1 (SL0505R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution; Predicted band size : 91kDObserved band size : 91kDWe are unsure as to the identity of these extra bandsSample:molt-4(human)cell Lysate at 40 ugPrimary: Anti- Sodium hydrogen exchanger 1 (SL0505R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 91kDObserved band size: 91 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (SL0505R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]

Function:
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Subunit:
Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic SLCterminus domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Kidney and intestine.

Post-translational modifications:
O-glycosylated.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1.

Similarity:
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

SWISS:
P19634

Gene ID:
6548

Database links:

Entrez Gene: 6548 Human

Entrez Gene: 20544 Mouse

Entrez Gene: 24782 Rat

Omim: 107310 Human

SwissProt: P19634 Human

SwissProt: Q61165 Mouse

SwissProt: P26431 Rat

Unigene: 469116 Human

Unigene: 4312 Mouse

Unigene: 5025 Rat

通道蛋白（Channel Protein）
钠-氢交换蛋白（NHE： Na+/H+ Exchanger）是维持细胞内环境的主要细胞膜蛋白质之一，它的一个主要功能是调节细胞内pH值，使之保持在生理范围之内。细胞内pH值的变化，直接或间接的影响细胞的生长分裂、增殖和分化，也影响细胞的死亡。
钠-氢交换蛋白质的活性还影响细胞的移动和肿瘤细胞的转移。
目前主要方向是：研究消化道肿瘤细胞转移。 Picture

Sample:
molt-4(human)cell Lysate at 40 ug
Primary: Anti- Sodium hydrogen exchanger 1 (SL0505R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (SL0505R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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