Rabbit Anti-SP7 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-SP7 antibody

SP7_HUMAN; Transcription factor Sp7; OSX; Zinc finger protein osterix; Sp7 transcription factor; Osterix;

Cat:

SL1110R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human SP7:331-431/431

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Spinal cord (Mouse) Lysate at 40 ugPrimary: Anti-Osterix(SL1110R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45kDObserved band size: 50kDSample:Embryo(Mouse) lysate at 30ug; Embryo(Mouse) lysate at 30ug; Primary: Anti-Osterix (SL1110R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 45kDObserved band size : 45kD Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (SL1110R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]

Function:
Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/SLCrich sequences (By similarity).

Subunit:
Interacts with NO66; the interaction is direct and inhibits transcription activator activity.

Subcellular Location:
Nucleus.

Tissue Specificity:
Osteoblast/chondrocyte specific.

DISEASE:
Osteogenesis imperfecta 12 (OI12) [MIM:613849]: A form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI12 is an autosomal recessive form characterized by recurrent fractures, mild bone deformations, generalized osteoporosis, delayed teeth eruption, no dentinogenesis imperfecta, normal hearing, and white sclerae. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
Q8TDD2

Gene ID:
121340

Database links:

Entrez Gene: 121340 Human

Entrez Gene: 170574 Mouse

Entrez Gene: 300260 Rat

Omim: 606633 Human

SwissProt: Q8TDD2 Human

SwissProt: Q5RM08 Mouse

SwissProt: Q8VI67 Mouse

SwissProt: Q6IMK1 Rat

Osterix成骨相关转录因子是一种具有锌指基序结构域的转录因子，在体内的表达对成骨细胞具有特异性。该蛋白只在发育的骨组织中特异性表达，是成骨细胞分化和骨形成过程中所必需的转录因子。骨髓基质干细胞分化为表达典型的成骨性标志基因的成骨细胞需要OSX的调控。 Picture

Sample:
Embryo(Mouse) lysate at 30ug;
Embryo(Mouse) lysate at 30ug;
Primary: Anti-Osterix (SL1110R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 45kD
Observed band size : 45kD

Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (SL1110R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall

Specific References (24) | SL1110R has been referenced in 24 publications.

[IF=2.1] Bo Zhang. et al. Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism. Mol Med Rep. 2020 Nov;22(5):4376-4382 WB ; Mouse.

PubMed:33000198

[IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039 IF,IHC ; Mouse.

PubMed:33511306

[IF=0] Bihari C et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. (2018) Hepatol Commun.Sep 4;2(9):1095-1110. IHC ; Human.

PubMed:30202823

[IF=0.57] Shi, Hua-Li, et al. "Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells." Tropical Journal of Pharmaceutical Research 16.7 (2017): 1595-1600. WB ; Human.

PubMed:10.4314/tjpr.v16i7.18

[IF=2.33] Qian, Dong-Yang, et al. "Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells." Biomedicine & Pharmacotherapy 90 (2017): 492-499. WB ; Mouse.

PubMed:28395271

[IF=1.56] Wang, Yilin, et al. "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." Molecular Medicine Reports (2016): 11. WB ; Rabbit.

PubMed:27878265

[IF=5.23] Li, Shao, et al. "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." Scientific Reports 7 (2017): 40505. WB ; Mouse.

PubMed:2808889

[IF=3.42] Tang, Zhurong, et al. "Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice." Materials Science and Engineering: C (2016). IHSLCP ; Mouse.

PubMed:27772699

[IF=0] Schlie-Wolter, S., et al. Osteogenic Differentiation of Human Adipose-Derived Stem Cells (hASC): Role of FAK and Ras-MAPK Signaling. (2016) J Stem Cell Regen Bio 2(2): 1- 19 WB ; Human.

PubMed:DOI:10.15436/2471-0598.16.012

[IF=2.2] Ranganathan, Kavitha, et al. "The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation." Journal of Surgical Research 206.1 (2016): 53-61. IF(IHSLCP) ; Mouse.

PubMed:27916375

[IF=6.52] Agarwal, Shailesh, et al. "Analysis of bone‐cartilage‐stromal progenitor populations in trauma induced and genetic models of heterotopic ossification." STEM CELLS (2016). IHSLCP ; Mouse.

PubMed:27068890

[IF=3.68] Dong, Weijie, et al. "Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance." Cell and Tissue Research (2014): 1-11. WB ; Rat.

PubMed:24927919

[IF=6.576] Kohei Arakawa. et al. The difference in joint instability affects the onset of cartilage degeneration or subchondral bone changes. Osteoarthr Cartilage. 2021 Dec;: IHC ; Mouse.

PubMed:34906679

[IF=2.626] Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUSLCMSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15 WB,IHC ; Mouse,Human.

PubMed:34778905

[IF=4.165] Yu-Ting Yen. et al. PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation. Commun Biol. 2021 Jun;4(1):1-12 WB,IHC ; Mouse.

PubMed:34079065

[IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;: IF,IHC ; Mouse.

PubMed:33711525

[IF=3.347] Yongkui Wang. et al. Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling. Toxicol Appl Pharm. 2020 Dec;409:115333 WB,IHC ; Rat, Mouse.

PubMed:33171191

[IF=3.448] Chang Z et al. Reticulocalbin 2 enhances osteogenic differentiation of human vascular smooth muscle cells in diabetic conditions. Life Sci. 2019 Sep 15;233:116746. WB ; Human.

PubMed:31401313

[IF=5.638] Cui Q et al. Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway. Cell Death Dis. 2019 Feb 20;10(3):172. WB ; Mouse.

PubMed:30787286

[IF=6.28] Chen et al. Diet derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice. (2013) J.Bone.Miner.Res. WB ; Mouse.

PubMed:23832484

[IF=5.587] Agarwal et al. Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. (2016) Stem.Cells. 34:1692-701 IF ; Human,Mouse.

PubMed:27068890

[IF=5.3] Rajshankar, Dhaarmini, Yongqiang Wang, and Christopher A. McCulloch. "Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts." The FASEB Journal (2016): fj-201600645R. WB ; Mouse.

PubMed:27881487

[IF=5.23] Agarwal, Shailesh, et al. "Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury." Scientific Reports 6 (2016): 32514. WB ; Mouse.

PubMed:27616463

[IF=2.86] Kishi, Yuta, et al. "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway."International Journal of Molecular Sciences 16.10 (2015): 24820-24838. WB ; Mouse.

PubMed:26492236