Rabbit Anti-PDGF-B antibody

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PDGF beta; PDGF-B; Platelet-derived growth factor B-chain; PDGF AB + BB; Becaplermin; c sis; FLJ1285; Oncogene SIS; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B chain; PDGF subunit A; PDGF subunit B; PDGF1; PDGF2; PDGFA; PDGFB; PDGFR1; Platelet derived gr

Cat:

SL0185R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PDGF-B:155-210/241

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: Lung (Mouse) Lysate at 40 ugLane 5: Lung (Rat) Lysate at 40 ugLane 6: Huvec (Human) Cell Lysate at 30 ugLane 7: A431 (Human) Cell Lysate at 30 ugLane 8: MCF-7 (Human) Cell Lysate at 30 ugLane 9: U2os (Human) Cell Lysate at 30 ugPrimary: Anti-PDGF-B (SL0185R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 28 kDSample: Stomach (Rat) Lysate at 40 ugPrimary: Anti-PDGF-B (SL0185R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 27 kDObserved band size: 32 kDTissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated(SL0071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control (blue line):Hela(blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (SL0185R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. [SUBUNIT] Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. Belongs to the PDGF/VEGF growth factor family.

Function:
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

Subunit:
Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1.

Subcellular Location:
Secreted. Note=Released by platelets upon wounding.

Tissue Specificity:
Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.

DISEASE:
Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P01127

Gene ID:
5155

Database links:

Entrez Gene: 5155 Human

Entrez Gene: 18591 Mouse

Entrez Gene: 24628 Rat

Omim: 190040 Human

SwissProt: P01127 Human

SwissProt: P3148 Mouse

SwissProt: Q05028 Rat

Unigene: 1976 Human

Unigene: 18889 Mouse

Unigene: 198230 Rat

生长因子和激素（ Growth Factor and Hormones）
PDGF（platelet-dirived growth factor）是一种碱性蛋白，分为两个亚基（A、B链）通过二硫键结合而成的两条多肽链的异二聚体，分子量为28-35kDa。为促细胞分裂剂，可使小鼠成纤维细胞及其他多种细胞增殖。最新研究发现了PDGF家族两种新的基因PDGF-C、PDGF-D。
Picture

Sample:
Stomach (Rat) Lysate at 40 ug
Primary: Anti-PDGF-B (SL0185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD

Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated(SL0071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control (blue line):Hela(blue).
Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (SL0185R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (8) | SL0185R has been referenced in 8 publications.

[IF=3.26] Hurley, Marja M., et al. "Accelerated Fracture Healing in Transgenic Mice Overexpressing an Anabolic Isoform of Fibroblast Growth Factor 2." Journal of Cellular Biochemistry (2015). IHSLCP ; Mouse.

PubMed:26252425

[IF=2.766] Wang et al. Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model. (2017) PLoS.One. 12:e0178619 WB ; Mouse.

PubMed:28570599

[IF=4.498] Furube et al. VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice. (2014) J.Endocrino. 222:161-79 WB ; Mouse.

PubMed:24860149

[IF=5.62] He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015). WB ;

PubMed:26272182

[IF=2.583] Du L et al. A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. Biomed Res Int. 2018 May 13;2018:1761865. WB ; Human.

PubMed:29862255

[IF=0] Almahrog et al. In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications. (2016) J.Oral.Biol.Craniofac.Res. 6:10-7 IHC ; Rat.

PubMed:26937363

[IF=2.77] Lee, Si‐Hyung, et al. "Therapeutic efficacy of autologous platelet‐rich plasma and polydeoxyribonucleotide on female pattern hair loss." Wound Repair and Regeneration (2014). WB ;

PubMed:2554827

[IF=3.33] Morita, Shoko, et al. "Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain." Cell and Tissue Research (2015): 1-20. IHSLCF ; Mouse.

PubMed:25573819