Rabbit Anti-Urokinase antibody

Home > Product > Antibody > Rabbit Anti-Urokinase antibody

Urokinase; ATF; ATF uPA; PLAU; UPA; Plasminogen activator; Plasminogen activator urinary; Plasminogen activator urokinase; u PA; U plasminogen activator; UPA; URK; Urokinase plasminogen activator; Urokinase type plasminogen activator; Urokinase type plasm

Cat:

SL220R

Species Reactivity：

Mouse,Rat,(predicted: Human,)

Immunogen：

KLH conjugated synthetic peptide derived from human PLAU:361-432/432

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ugLane 2: Liver (Mouse) Lysate at 40 ugLane 3: Lung (Mouse) Lysate at 40 ugLane 4: Spleen (Mouse) Lysate at 40 ugLane 5: Liver (Rat) Lysate at 40 ugLane 6: Lung (Rat) Lysate at 40 ugLane 7: Spleen (Rat) Lysate at 40 ugPrimary: Anti-Urokinase (SL220R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40-80 kDObserved band size: 45 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Urokinase (SL220R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49 kDSample: Cerebrum (mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-Urokinase (SL220R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49kDObserved band size: 48kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PLAU/UPA/Urokinase Polyclonal Antibody, Unconjugated(SL220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Urokinase is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in the Urokinase gene may be associated with late onset Alzheimer disease and also with decreased affinity for fibrin binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg Val bond in plasminogen. The proprotein is cleaved at a Lys Ile bond by plasmin to form a two chain derivative in which a single disulfide bond connects the amino terminal A chain to the catalytically active, carboxy terminal B chain. This two chain derivative is also called HMW uPA (high molecular weight uPA). HMW uPA can be further processed into LMW uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino terminal fragment. LMW uPA is proteolytically active but does not bind to the uPA receptor.

Function:
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in the prostate gland and prostate cancers.

Post-translational modifications:
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Similarity:
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.

SWISS:
P00749

Gene ID:
5328

Database links:

Entrez Gene: 5328 Human

Entrez Gene: 18792 Mouse

Entrez Gene: 25619 Rat

Omim: 191168 Human

SwissProt: P00749 Human

SwissProt: P06869 Mouse

SwissProt: P29598 Rat

Unigene: 77274 Human

Unigene: 4183 Mouse

Unigene: 6064 Rat

尿激酶型纤溶酶激活因子;是一种多效细胞因子，参与多种病理生理过程；uPA系统可通过调节纤溶酶的活性降解p淀粉样蛋白;是一种丝氨酸蛋白酶，将血浆酶原转化成血浆酶（纤维蛋白溶酶）。 Picture

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Urokinase (SL220R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD

Sample:
Cerebrum (mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Urokinase (SL220R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 48kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PLAU/UPA/Urokinase Polyclonal Antibody, Unconjugated(SL220R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall