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Home > Product > Antibody > Rabbit Anti-TGF beta 2 Propeptide antibody
Transforming growth factor beta-2 proprotein; TGFB2; TGF B2; BSC 1 cell growth inhibitor; BSC-1 cell growth inhibitor; BSC1 cell growth inhibitor; Cetermin; G TSF; G-TSF; Glioblastoma derived T cell suppressor ; Glioblastoma derived T cell suppressor fact
Cat:
SL0100R
Species Reactivity：
Human,Mouse,(predicted: Rat,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human TGF-beta 2:154-197/414
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample:Kidney(Mouse)Lysate at 40 ugPrimary: Anti-TGF beta 2 Propeptide(SL0100R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 12'45kDObserved band size: 50 kDSample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Lung tissue lysates Primary: Anti- TGF beta 2 Propeptide (SL0100R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12/45 kDaObserved band size: 48 kDaSample:MCF-7 (Human)Cell Lysate at 40 ugPrimary: Anti-TGF beta 2 Propeptide(SL0100R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 12'45kDObserved band size: 48kDTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated(SL0100R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Datasheet:


Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

Function:
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Subunit:
Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN.

Subcellular Location:
Secreted.

Similarity:
Belongs to the TGF-beta family.

SWISS:
P61812

Gene ID:
7042

Database links:

Entrez Gene: 7042 Human

Entrez Gene: 2368 Mouse

Omim: 190220 Human

SwissProt: P21214 Cow

SwissProt: P61812 Human

SwissProt: P27090 Mouse

Unigene: 133379 Human

Unigene: 18213 Mouse



生长因子和激素（ Growth Factor and Hormones）
TGF是一种多效生长因子,对上皮细胞增殖有潜在抑制作用,可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。此抗体是TGFβ2 特异性抗体，与TGF-β1和TGF-β3没有交叉反应。

Picture

Sample:Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(SL0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti- TGF beta 2 Propeptide (SL0100R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/45 kDa
Observed band size: 48 kDa
Sample:MCF-7 (Human)Cell Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(SL0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 48kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated(SL0100R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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