Rabbit Anti-TSLC1 antibody | Sunlong Medical

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CADM1; BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin Superfamily Member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily;

Cat:

SL1147R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human TSLC1:365-442/442<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Testis (Mouse) Lysate at 40 ugLane 3: U2os (Human) Cell Lysate at 30 ugLane 4: 293T (Human) Cell Lysate at 30 ugPrimary: Anti-TSLC1 (SL1147R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 100 kDTissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(SL1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(SL1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Function:
Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Subunit:
Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its SLCterminus with the PDZ domain of MPP3 and the PDZ domain of MPP6.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.

Similarity:
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q9BY67

Gene ID:
23705

Database links:

Entrez Gene: 23705 Human

Entrez Gene: 54725 Mouse

Entrez Gene: 363058 Rat

Omim: 605686 Human

SwissProt: Q9BY67 Human

SwissProt: Q8R5M8 Mouse

Unigene: 370510 Human

Unigene: 234832 Mouse

TSLC1属于新的抑癌基因免疫球蛋白超家族, TSLC1蛋白质参与细胞间黏附、细胞运动、信号转导及免疫调节. TSLC1在多种肿瘤中表达异常,它对肿瘤的影响主要表现为抑制瘤细胞增殖及诱导凋亡、改变瘤细胞的生长特性及基因表达,可抑制上皮间质转化.
TSLC1蛋白在控制肿瘤生物侵袭性方面有着重要作用，也可能作为预测患者预后的重要生物标记，但是TSLC1的肿瘤抑制分子机制还有待进一步研究。 Picture

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(SL1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(SL1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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