Rabbit Anti-Nogo B antibody

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reticulon-4 isoform B; RTN4; RTN4-B1; Nbla10545; RTN-X; reticulon-4A; Nogo-C; NSP; RTN-x; ASY; foocen; NSP-CL; NOGOC; RTN4-A; Reticulon-4; Nbla00271; KIAA0886; NOGOA; Reticulon-5; Foocen; NI220/250; RTN4-C; RTN4-B2; SP1507; NOGO-A; NOGOB; Nogo-B; Nogo-B/A

Cat:

SL1315R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RTN4 isoform B:251-357/357

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Cerebellum (Mouse) Lysate at 40 ugLane 2: Cerebellum (Rat) Lysate at 40 ugPrimary: Anti-Nogo B (SL1315R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 45 kDSample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti-Nogo A+B (SL1315R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39 kDObserved band size: 39 kDTissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Nogo-B Polyclonal Antibody, Unconjugated(SL1315R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control (blue line): A431(blue). Primary Antibody (green line): Rabbit Anti-Nogo A+B/PE-CY5 Conjugated antibody (SL1315R-PE-CY5) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5 . ProtocolThe cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.

Function:
NOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (15992), and Nogo. Several isoforms (A-E) of NOGO exist.

Subcellular Location:
Endoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.

SWISS:
Q9NQC3

Gene ID:
57142

Database links:

Entrez Gene: 57142 Human

Entrez Gene: 68585 Mouse

Entrez Gene: 83765 Rat

Omim: 604475 Human

SwissProt: Q9NQC3 Human

SwissProt: Q99P72 Mouse

SwissProt: Q9JK11 Rat

Unigene: 704007 Human

神经生物学相关蛋白（Neurobiology）
Nogo-B蛋白是一组参与组织损伤后再生的跨膜功能蛋白，其中Nogo-B位于多种组织器官中而发挥不同的功能。目前研究发现，Nogo-B与肿瘤抑制、凋亡、神经系统再生抑制、血管内膜损伤修复等多种病理生理过程有不同程度的关系. Picture

Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Nogo A+B (SL1315R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Nogo-B Polyclonal Antibody, Unconjugated(SL1315R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control (blue line): A431(blue).
Primary Antibody (green line): Rabbit Anti-Nogo A+B/PE-CY5 Conjugated antibody (SL1315R-PE-CY5)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5 .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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