Rabbit Anti-Neurogenin3 antibody

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NGN3; NGN-3; neurogenin 3; Neurog3; Neurogenin-3; ATH4B; Atoh5; Math4B; NEUROG3; ngn3; OTTHUMP00000019730; Protein atonal homolog 5; Class A basic helix-loop-helix protein 7; NGN3_HUMAN.

Cat:

SL0922R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NGN3:61-160/214

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Liver(Mouse) Lysate at 30 ugPrimary: Anti-Neurogenin3 (SL0922R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 26 kDParaformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurogenin 3) Polyclonal Antibody, Unconjugated (SL0922R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated(SL0922R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm.

Function:
Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.

Subcellular Location:
Nucleus.

DISEASE:
Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4) [MIM:610370]. DIAR4 is a characterized by severe, life-threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
Q9Y4Z2

Gene ID:
50674

Database links:

Entrez Gene: 50674 Human

Entrez Gene: 11925 Mouse

Entrez Gene: 60329 Rat

Omim: 604882 Human

SwissProt: Q9Y4Z2 Human

SwissProt: P70661 Mouse

Unigene: 532682 Human

Unigene: 57236 Mouse

神经生物学相关蛋白（Neurobiology）
Ngn3属于bHLH家族蛋白，是胰腺发育过程中短暂表达的蛋白，可能是胰腺内分泌细胞系的定向因子，一般在成熟胰岛中不存在。
Ngn3的表达会导致胰腺内分泌细胞数的增加外分泌细胞数的减少。因而胰岛β细胞并不是完全靠干细胞分化产生，主要是通过已分化胰岛β细胞的分裂增殖。
但近年来，有学者认为：Ngn3虽在胰腺发育中短暂表达，但是否存在使胰岛干细胞或前体细胞自我更新的能力尚未明确定论。 Picture

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurogenin 3) Polyclonal Antibody, Unconjugated (SL0922R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated(SL0922R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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