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Home > Product > Antibody > Rabbit Anti-Integrin alpha 5 antibody
Alpha 5 beta 1; CD49 antigen-like family member E; CD49e; ECMRVI; Fibronectin Receptor alpha; Fibronectin receptor alpha subunit; Fibronectin receptor, alpha subunit; FNR; FNRA; gpIc/IIa; Integrin alpha 5 (fibronectin receptor alpha); Integrin alpha F; In
Cat:
SL0567R
Species Reactivity：
Human,Mouse,(predicted: Rat,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human Integrin alpha-5 light chain:1021-1099/1099<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Product PDFs
Datasheet:


The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Function:
CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5); in platelets as glycoprotein IcIIa; and in fibroblasts as extracellular matrix receptor 6 (ECMVI). In addition to adhesion, integrins are known to participate in cell surface mediated signalling.

Subunit:
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HISLV1 Tat.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.

SWISS:
P08648

Gene ID:
3678

Database links:

Entrez Gene: 3678 Human

Entrez Gene: 11282 Mouse

Entrez Gene: 315346 Rat

Omim: 135620 Human

SwissProt: P08648 Human

SwissProt: P11688 Mouse

Unigene: 505654 Human

Unigene: 16234 Mouse

Unigene: 100796 Rat



ITG-α5粘附分子整合素α5也是细胞粘附分子的超家族成员，广泛分布于细胞表面和细胞外基质的糖蛋白,不仅介导细胞与细胞或细胞与基质之间的连接,而且参与细胞间的信号转导,细胞的生长移行,肿瘤的转移浸润,免疫炎性反应,血管生成等一系列的生理和病理过程。 Picture

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Integrin alpha 5 Polyclonal Antibody, Unconjugated(SL0567R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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