VASH1 is a angiogenesis inhibitor. It inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. It does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis and acts in an autocrine manner. VASH1 also inhibits artery neointimal formation and macrophage infiltration and exhibits heparin-binding activity. There are two named isoforms.
Function:
Angiogenesis inhibitor. Inhibits migration,proliferation and network formation by endothelial cells as well asangiogenesis. This inhibitory effect is selective to endothelialcells as it does not affect the migration of smooth muscle cells orfibroblasts. Does not affect the proliferation of cancer cells invitro, but inhibits tumor growth and tumor angiogenesis. Acts in anautocrine manner. Inhibits artery neointimal formation andmacrophage infiltration. Exhibits heparin-binding activity.
Subcellular Location:
Secreted.
Tissue Specificity:
Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
Post-translational modifications:
2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa)may be processed by proteolytic cleavage. The largest form (42 kDa)seems to be secreted and the other major form (63 kDa) seems toaccumulate within the cells or pericellular milieu. Polypeptideconsisting of Met-77 to Arg-318 may correspond to the 27 kDa formand that consisting of Met-77 to Val-365 may correspond to the 36kDa form.
Similarity:
Belongs to the vasohibin family.
SWISS:
Q7L8A9
Gene ID:
22846
Database links:
Entrez Gene: 22846 Human
Entrez Gene: 238328 Mouse
Entrez Gene: 503052 Rat
Omim: 609011 Human
SwissProt: Q7L8A9 Human
SwissProt: Q8C1W1 Mouse
Unigene: 525479 Human
Unigene: 216101 Mouse
VASH1是一个内皮组织衍生的可被血管内皮生长因子诱导的分泌性蛋白质,它是目前为止发现的唯一以负反馈为作用机制调节血管新生的因子。它的发现证实在血管新生的过程中也存在经典的自身调节控制机制—负反馈调节机制的存在。
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Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- Vasohibin 1 (SL1505R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample: Liver（Rat） lysate at 30ug;
Primary: Anti-alpha-Actinin-4 (SL1505R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1:3000 dilution;
Predicted band size : 40kD
Observed band size : 40kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min;
Incubation: Anti-VASH1 Polyclonal Antibody, Unconjugated(SL1505R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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