Rabbit Anti-VCAM1 antibody | Sunlong Medical

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VCAM1; CD 106; CD106; CD106 Antigen; DKFZp779G2333; INCAM 100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; INCAM-100; V-CAM 1; VCAM1_HUMAN.

Cat:

SL0396R

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse VCAM-1:640-739/739<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1µg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Siha (Human) Cell Lysate at 30 ugLane 2: Huvec (Human) Cell Lysate at 30 ugLane 3: U251 (Human) Cell Lysate at 30 ugPrimary: Anti-VCAM-1 (SL0396R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110 kDObserved band size: 110 kDSample: VCAM1 protein (Human) at 100 ngPrimary: Anti-VCAM-1 (SL0396R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated(SL0396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: Mouse Spleen(blue). Primary Antibody:Rabbit Anti-VCAM-1 antibody (SL0396R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0396R, 1μg /8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.

Function:
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subunit:
Binds to ECMSLVD capsid proteins and acts as a receptor for this virus.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow.

Similarity:
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P29533

Gene ID:
22329

Database links:

Entrez Gene: 7412 Human

Entrez Gene: 22329 Mouse

Entrez Gene: 25361 Rat

GenBank: NP_001069.1 Human

GenBank: NP_001186763.1 Human

Omim: 192225 Human

SwissProt: P1964 Human

SwissProt: P29533 Mouse

VCAM 1（CD106）是一种膜相关蛋白，分布于血管内皮及其周围的淋巴细胞、单核细胞、嗜酸性粒细胞和中性粒细胞。Anti-VCAM-1主要用于各种组织中血管内皮的检测。 Picture

Sample: VCAM1 protein (Human) at 100 ng
Primary: Anti-VCAM-1 (SL0396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated(SL0396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: Mouse Spleen(blue).
Primary Antibody:Rabbit Anti-VCAM-1 antibody (SL0396R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0396R, 1μg /8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (12) | SL0396R has been referenced in 12 publications.

[IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025 IF ; Rat.

PubMed:35026282

[IF=3.241] Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019 WB ; Mouse.

PubMed:31246210

[IF=5.646] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55. FCM&IHC ; Mouse&Human.

PubMed:30728051

[IF=2.976] Peng Y et al. Sonodynamic therapy improves anti‑tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft. Oncol Rep. 2018 Oct;40(4):2163-2170. IHF ; Mouse.

PubMed:30106435

[IF=16.834] Moccetti F et al. Myocardial Infarction Produces Sustained Proinflammatory Endothelial Activation in Remote Arteries. J Am Coll Cardiol. 2018 Aug 28;72(9):1015-1026. IHF ; Mouse.

PubMed:30139430

[IF=5.151] Zhang L et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice.Mol Nutr Food Res. 2018 Aug;62(16):e360315. WB ; Mouse.

PubMed:29883529

[IF=4.932] Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096 WB ; Human.

PubMed:34464886

[IF=3.968] Wei Tang. et al. Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states. Asian J Pharm Sci. 2021 Apr;: WB ; Human.

PubMed:10.1016/j.ajps.2021.03.005

[IF=4.545] Chae Eun Kim. et al. Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model. Biomed Pharmacother. 2021 Jul;139:111571 IHC ; Mouse.

PubMed:33857915

[IF=2.314] Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757. WB ; Mouse.

PubMed:30739356

[IF=3.47] Ding L et al. Saponins of sea cucumber attenuate atherosclerosis in ApoE −/− mice via lipid-lowering and anti-inflammatory properties. Journal of Functional Foods,2018 48, 490–497. WB ; Rat&Mouse.

PubMed:10.1016/j.jff.2018.07.046

[IF=3.97] Ding, Lin, et al. "Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism." Journal of Functional Foods 32 (2017): 90-97. WB ; Mouse.

PubMed:doi:10.1016/j.jff.2017.02.020