Rabbit Anti-E cadherin antibody

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E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion mo

Cat:

SL1519R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human E-cadherin:841-882/882<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugHepG2(Human) Cell Lysate at 30 ugPrimary: Anti- E cadherin (SL1519R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 90/97 kDObserved band size: 125 kDSample: Lane 1: MDA-MB-231 (Human) Cell Lysate at 30 ugLane 2: A431 (Human) Cell Lysate at 30 ugLane 3: A549 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti- E cadherin (SL1519R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 125 kDObserved band size: 120 kD Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL1519R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (E cadherin) polyclonal Antibody, Unconjugated (SL1519R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit:
Homodimer.

Subcellular Location:
Cell junction. Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Non-neural epithelial tissues.

Post-translational modifications:
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.

DISEASE:
Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.
Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).
Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].
Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.

Similarity:
Contains 5 cadherin domains.

SWISS:
P12830

Gene ID:
999

Database links:

Entrez Gene: 999 Human

Entrez Gene: 12550 Mouse

Entrez Gene: 83502 Rat

Omim: 192090 Human

SwissProt: P12830 Human

SwissProt: P01963 Mouse

SwissProt: Q9R0T4 Rat

Unigene: 461086 Human

Unigene: 35605 Mouse

Unigene: 1303 Rat

细胞粘附蛋白（Call Adhesion Protein）
E-chaherin是研究较多的同质粘附分子。E-cadherin的表达与恶性肿瘤的分化程度、侵袭力、转移负相关与预后正相关，E-cadherin的低表达和不稳定表达可促进转移的发生。近年来已成为肿瘤细胞侵袭和转移研究的热点之一。 Picture

Sample:
Lane 1: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Primary:
Anti- E cadherin (SL1519R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 120 kD

Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (SL1519R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Tissue/cell: A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (E cadherin) polyclonal Antibody, Unconjugated (SL1519R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

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Specific References (16) | SL1519R has been referenced in 16 publications.

[IF=2.344] Zhu, Ting. et al. Irisin/FNDC5 inhibits the epithelial–mesenchymal transition of epithelial ovarian cancer cells via the PI3K/Akt pathway. Arch Gynecol Obstet. 2022 Feb;:1-10 WB ; Human.

PubMed:35156135

[IF=6.543] Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022 WB,IF ; Rat,Human.

PubMed:35308169

[IF=5.81] Zhou Z. et al. Vanillin Derivatives Reverse Fusobacterium nucleatum-Induced Proliferation and Migration of Colorectal Cancer Through E-Cadherin/β-Catenin Pathway.. Front Pharmacol. 2022 Mar;13:841918-841918 WB ; Human.

PubMed:35308221

[IF=4.784] Zheng Wu. et al. FOXD3 suppresses epithelial–mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22 WB ; human.

PubMed:34551139

[IF=3.481] Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25 WB ; Human.

PubMed:34032284

[IF=4.175] Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15 WB ;

PubMed:33407505

[IF=6.491] Nikhil K et al. Identification of LIMK2 as a Therapeutic Target in Castration Resistant Prostate Cancer. Cancer Lett. 2019 Apr 28;448:182-196. WB&IHC ; Mouse.

PubMed:3071672

[IF=3.333] Liu SQ et al. Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.Int. J. Oncol. 2018 Oct 25 IHC ; Human.

PubMed:30365116

[IF=2.15] Fan, Hai-Xia, et al. "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." Medical Oncology 31.7 (2014): 1-8. IHSLCP ; Human.

PubMed:24915900

[IF=1.61] Yu, Yang, et al. "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells." Acta Histochemica (2014). WB ; Human.

PubMed:24681113

[IF=6.244] Pei Dai. et al. Gimap5 Inhibits Lung Cancer Growth by Interacting With M6PR. Front Oncol. 2021; 11: 699847 WB ; Human.

PubMed:3924035

[IF=11.161] Zhipeng Jiang. et al. EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway. J Exp Clin Canc Res. 2021 Dec;40(1):1-15 WB,IHC ; Human.

PubMed:34253241

[IF=4.175] Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12 WB ; Human.

PubMed:33292220

[IF=1.977] Qinhong Xu. et al. Overexpression of circ_0001445 decelerates hepatocellular carcinoma progression by regulating miR-942-5p/ALX4 axis. Biotechnol Lett. 2020 Dec;42(12):2735-2747 WB ; Human.

PubMed:32856218

[IF=3.405] Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. WB ; Human.

PubMed:30899449

[IF=3.7] Wang et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. (2018) Cancer.Manag.Res. 10:1479-1486 IHC ; Human.

PubMed:29928144