Rabbit Anti-Calpain 2 antibody

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Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; CALP80; Calpain 2 (m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; Ca

Cat:

SL1599R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse Calpain 2:301-400/700

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-Calpain 2 (SL1599R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 78 kDSample: Brain (Mouse) Lysate at 40 ugPrimary: Anti- Calpain 2(SL1599R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 78 kDObserved band size: 90 kDParaformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpain 2) Polyclonal Antibody, Unconjugated (SL1599R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.
Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Function:
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Subunit:
Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Subcellular Location:
Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 2 EF-hand domains.

SWISS:
O08529

Gene ID:
12334

Database links:

Entrez Gene: 824 Human

Entrez Gene: 12334 Mouse

Entrez Gene: 29154 Rat

Omim: 114230 Human

SwissProt: P17655 Human

SwissProt: O08529 Mouse

SwissProt: Q07009 Rat

Unigene: 350899 Human

Unigene: 19306 Mouse

Unigene: 6822 Rat

钙蛋白酶2(calpain2)也是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括细胞骨架和细胞膜附着的构型重建、不同的信号转导途径和细胞凋亡等. Picture

Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Calpain 2(SL1599R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 90 kD

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calpain 2) Polyclonal Antibody, Unconjugated (SL1599R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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