Rabbit Anti-CX3CR1 antibody

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Beta chemokine receptor-like 1; C X3 C CKR 1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMKBLR1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; Fractalkine Receptor; GPR13; GPRV28; V28; Chemokine receptor 1(chemokine (C-X3-C motif) r

Cat:

SL1728R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CX3CR1:151-250/355<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/Test（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Lymph node (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Cerebrum (Mouse) Lysate at 40 ugLane 4: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CX3CR1 (SL1728R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50/55 kDObserved band size: 50 kDSample: Heart(Mouse) Lysate at 40 ugHeart(Rat) Lysate at 40 ugPrimary: Anti-CX3CR1 (SL1728R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50'55 kDObserved band size: 53 kDBlank control:Raw264.7. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 0.5ug/Test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HISLV1, and some variations in this gene lead to increased susceptibility to HISLV1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Function:
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HISLV1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HISLV1 coreceptors than isoform 1.

Subunit:
Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response. Interacts with HISLV1 envelope polyprotein gp160.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in lymphoid and neural tissues.

DISEASE:
Age-related macular degeneration 12 (ARMD12) [MIM:613784]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P49238

Gene ID:
1524

Database links:

Entrez Gene: 1524 Human

Entrez Gene: 13051 Mouse

Entrez Gene: 171056 Rat

SwissProt: P49238 Human

SwissProt: Q9Z0D9 Mouse

SwissProt: P35411 Rat

Picture

Sample:
Heart(Mouse) Lysate at 40 ug
Heart(Rat) Lysate at 40 ug
Primary: Anti-CX3CR1 (SL1728R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50'55 kD
Observed band size: 53 kD

Blank control:Raw264.7.
Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody (SL1728R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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