Rabbit Anti-ECE2 antibody | Sunlong Medical

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ECE2; MGC78487; ECE 2; Endothelin converting enzyme 2; Hypothetical protein MGC2408; KIAA0604; MGC17664; ECE2_HUMAN.

Cat:

SL1753R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human ECE2:601-700/883

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T Cell (Human) Lysate at 40 ugPrimary: Anti-ECE2 (SL1753R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80-100 kDObserved band size: 80 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (SL1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (SL1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Endothelin-converting enzyme 2 (ECE2) converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Expressed at high levels in central nervous system. Expressed in adrenal glands, ovary and uterus, and at low levels in heart.

Subcellular Location:
Golgi apparatus membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic granule membrane; Single-pass type II membrane protein (By similarity).

Tissue Specificity:
Expressed in brain. Strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level).

Similarity:
In the N-terminal section; belongs to the methyltransferase superfamily.
In the SLCterminal section; belongs to the peptidase M13 family.

SWISS:
O60344

Gene ID:
9718

Database links:

Entrez Gene: 281134 Cow

Entrez Gene: 9718 Human

Omim: 610145 Human

SwissProt: Q10711 Cow

SwissProt: O60344 Human

Unigene: 146161 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (SL1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (SL1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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