Rabbit Anti-Elastin antibody

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alpha Elastin/Tropoelastin; Elastin isoform a; ELN; ELN_HUMAN; elastin isoform m precursor; FLJ38671; FLJ43523; Supravalvular aortic stenosis; Tropoelastin; Williams Beuren syndrome; Williams syndrome region; ADCL1; SVAS; WBS; WS.

Cat:

SL1756R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human Elastin:681-786/786

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Uterus (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Liver (Mouse) Lysate at 40 ugLane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 5: Lung (Rat) Lysate at 40 ugLane 6: Liver (Rat) Lysate at 40 ugLane 7: Testis (Rat) Lysate at 40 ugLane 8: SH-SY5Y (Human) Cell Lysate at 30 ugLane 9: A549 (Human) Cell Lysate at 30 ugPrimary: Anti-Elastin (SL1756R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 66 kDObserved band size: 68 kDSample: Lung (Mouse) Lysate at 30 ugPrimary: Anti- alpha Elastin/Tropoelastin (SL1756R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 70 kDObserved band size: 68 kDImages provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (SL1756R) at 1:1000 4℃ temperature overnight 4℃followed by conjugation to secondary antibody.The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (SL1756R)plus secondary antibody.Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (SL0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017].

Function:
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.

Subunit:
The polymeric elastin chains are cross-linked together into an extensible 3D network. Forms a ternary complex with BGN and MFAP2. Interacts with MFAP2 via divalent cations (calcium > magnesium > manganese) in a dose-dependent and saturating manner.

Subcellular Location:
Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.

Tissue Specificity:
Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.

DISEASE:
Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1) . A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.

Similarity:
Belongs to the elastin family.

SWISS:
P15502

Gene ID:
2006

Database links:

Entrez Gene: 2006 Human

Entrez Gene: 13717 Mouse

Omim: 130160 Human

SwissProt: P15502 Human

SwissProt: P5464 Mouse

Unigene: 647061 Human

Picture

Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- alpha Elastin/Tropoelastin (SL1756R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD

Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (SL1756R) at 1:1000 4℃ temperature overnight 4℃followed by conjugation to secondary antibody.

The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (SL1756R)plus secondary antibody.
Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (SL0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.

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