Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Inhibin beta B (SL1825R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Mouse Liver tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Breast tissue lysates
Lane 7: Human A431 cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- Inhibin beta B (SL1825R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/45 kDa
Observed band size: 46 kDa
Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti- Inhibin beta B (SL1825R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (SL1825R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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