Rabbit Anti-IL-11 antibody | Sunlong Medical

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Interleukin-11; Adipogenesis inhibitory factor; AGIF; IL 11; IL11; Interleukin 11; Oprelvekin; IL11_HUMAN.

Cat:

SL1827R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human IL-11:22-120/199

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 30 ugPrimary: Anti- IL-11 (SL1827R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 19 kDObserved band size: 19 kDSample: Lane 1: Mouse Kidney tissue lysates Lane 2: Rat Kidney tissue lysatesPrimary: Anti-IL-11 (SL1827R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 19 kDaObserved band size: 19 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (SL1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (SL1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.

Function:
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-6 superfamily.

SWISS:
P20809

Gene ID:
3589

Database links:

Entrez Gene: 3589 Human

Omim: 147681 Human

SwissProt: P20809 Human

Unigene: 467304 Human

白介素11，是一种多向性细胞因子。很多细胞都能合成IL11，如：内皮细胞、纤维母细胞、软骨细胞、滑膜细胞、成骨细胞、上皮细胞、神经元、胸腺等。正常的血清中，IL11表达很少或处在检测水平之下。对造血系统的功能包括：干细胞、巨核细胞、骨髓细胞、红细胞和B淋巴细胞的增生和发育。对造血系统之外也具有多相性的作用，有些是刺激和促进增生，有些是抑制作用。人、大鼠、小鼠的IL-11的前体有199个氨基酸。成熟形态为178个氨基酸。 Picture

Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-IL-11 (SL1827R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (SL1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (SL1827R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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