Rabbit Anti-WRCH1 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-WRCH1 antibody

WRCH-1; WRCH 1; Wnt-1 responsive Cdc42 homolog 1; Rho-related GTP-binding protein RhoU; Ryu GTPase; CDC42-like GTPase 1; Rho GTPase-like protein ARHU; GTP-binding protein-like 1; RHOU; SB128; ARHU; CDC42L1; G28K; WRCH1; RHOU_HUMAN.

Cat:

SL1944R

Species Reactivity：

Mouse,Rat,(predicted: Human,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human WRCH-1:151-258/258

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti- WRCH1 (SL1944R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The WRCH1 protein is a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation.

Function:
Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.

Subunit:
Interacts with PAK3.

Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction, focal adhesion. Cell projection, podosome. Note=Localizes to podosomes in SRSLCtransformed cells.

Tissue Specificity:
Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.

Post-translational modifications:
Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.

Similarity:
Belongs to the small GTPase superfamily. Rho family.

SWISS:
Q7L0Q8

Gene ID:
5896

Database links:

Entrez Gene: 5896 Human

Entrez Gene: 69581 Mouse

Omim: 606366 Human

SwissProt: Q7L0Q8 Human

SwissProt: Q9EQT3 Mouse

Unigene: 647774 Human

Unigene: 168257 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated (SL1944R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Feedback Wall