Rabbit Anti-IL-1R2 antibody

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IL1 Receptor II; IL-1R II; IL1 Receptor 2; interleukin-1 receptor type 2 precursor; IL1R2; CD121b; IL1RB; CD121; CD121 antigen-like family member B; CD121b; CD121B Antigen; CDW121B; IL-1R-2; IL-1R-beta; IL-1R2; IL-1RT-2; IL-1RT2; IL1R Beta; Il1r-2; IL1R2;

Cat:

SL2595R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human IL-1R II:301-398/398<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: SW96 Cell (Human) Lysate at 40 ugPrimary: Anti- IL-1R2 (SL2595R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 44 kD Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated (SL2595R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: RSC96 cells(blue). Primary Antibody: Rabbit Anti-IL-1R2 antibody(SL2595R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4(IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq].

Function:
Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Subcellular Location:
Isoform Short: Secreted.
Isoform Long: Cell membrane; Single-pass type I membrane protein.

Post-translational modifications:
A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported.

Similarity:
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P27930

Gene ID:
7850

Database links:

Entrez Gene: 7850 Human

Omim: 147811 Human

SwissProt: P27930 Human

Unigene: 25333 Human

IL-1RⅡ属于新的信号受体家族Toll信号转导通路蛋白，IL-1RⅡ在免疫和炎症反应的研究中占重要地位，是在很多感染、炎症性致病机制中起决定性作用的细胞因子，几乎可作用于全身各种细胞，IL-1 与IL-1RⅡ的结合可导致大量与免疫反应和炎症相关基因的表达，包括细胞因子、细胞因子受体、急性期反应物、生长因子、胞外基质、粘附分子等。IL-1RⅡ/NF-κB信号通路是机体免疫反应中十分重要的组成部分。 Picture

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated (SL2595R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: RSC96 cells(blue).
Primary Antibody: Rabbit Anti-IL-1R2 antibody(SL2595R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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