Rabbit Anti-CLEC5A antibody

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A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; C-type lectin domain family 5 member A; C type lectin domain family 5 member A; C-type lecti

Cat:

SL2663R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse CLEC5A:101-190/190<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-CLEC5A(SL2663R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDSample: Lane 1: Mouse Spinal cord tissue lysates Lane 2: Rat Spinal cord tissue lysates Primary: Anti-CLEC5A (SL2663R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDaObserved band size: 37 kDaParaformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLEC5A) Polyclonal Antibody, Unconjugated (SL2663R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated(SL2663R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingCell: U937.Concentration: 1:100.Incubation: 40 minutes, room temperature. Host/Blank: U937 cells. Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (SL2663R) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells. secondary antibody: Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC)

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the SLCtype lectin/SLCtype lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq].

Function:
Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through phosphylation of TYROBP, this interaction does not result in viral entry but stimulates proinflammatory cytokine release.

Subunit:
Monomer. Homodimer. The majority of CLEC5A is expressed as a monomeric form on macrophages. Interacts with TYROBP/DAP12. The interaction with TYROBP is required for CLEC5 cell surface expression. Interacts with HCST/DAP10. Forms an CLEC5A/TYROBP/HCST trimolecular complex depending almost solely on TYROBP.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Tissue Specificity:
Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U937 and MonoMac6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells.

Post-translational modifications:
N-glycosylated. Contains sialic acid residues.

DISEASE:
Note=Involved in the pathogenetic mechanisms of Japanese encephalitis virus (JEV) infection of the brain. JEV infection of young mice results in increased expression of CLEC5A in spleen and brain with consequent activation of proinflammatory cytokines secretion.

Similarity:
Contains 1 SLCtype lectin domain.

SWISS:
Q9R007

Gene ID:
23845

Database links:

Entrez Gene: 2721 Human

Entrez Gene: 23845 Mouse

Entrez Gene: 679787 Rat

Omim: 604987 Human

SwissProt: Q9NY25 Human

SwissProt: Q9R007 Mouse

Unigene: 446235 Human

Unigene: 103765 Mouse

Unigene: 218435 Rat

Picture

Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Rat Spinal cord tissue lysates
Primary: Anti-CLEC5A (SL2663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 37 kDa

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLEC5A) Polyclonal Antibody, Unconjugated (SL2663R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated(SL2663R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Cell: U937.
Concentration: 1:100.
Incubation: 40 minutes, room temperature.
Host/Blank: U937 cells.
Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (SL2663R) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells.
secondary antibody: Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC)

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