Rabbit Anti-PAX7 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-PAX7 antibody

Paired box gene 7; FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7B; RGD1564360; PAX7

Cat:

SL2413R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human PAX7:401-500/520

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2 (human)Cell Lysate at 40 ugPrimary: Anti-PAX7(SL2413R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDBlank control: A549. Primary Antibody (green line): Rabbit Anti-PAX7 antibody (SL2413R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of PAX7 is unknown but it is speculated to be involved in tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Function:
Probable transcription factor. May have a role in myogenesis.

Subunit:
Can bind to DNA as a heterodimer with PAX3. Interacts with DAXX.

Subcellular Location:
Nucleus.

DISEASE:
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.

Similarity:
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.

SWISS:
P23759

Gene ID:
5081

Database links:

Entrez Gene: 5081 Human

Entrez Gene: 18509 Mouse

Entrez Gene: 500574 Rat

Omim: 167410 Human

SwissProt: P23759 Human

SwissProt: P47239 Mouse

Unigene: 113253 Human

Unigene: 218760 Mouse

Unigene: 226327 Rat

同源结构域蛋白（Homeodomain Proteins）
PAX-7属于转录抑制因子。在胚胎发育和肿瘤生长中起关键作用。其突变和某些肿瘤的发病有关。 Picture

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-PAX7 antibody (SL2413R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall