Rabbit Anti-CD30 antibody | Sunlong Medical

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CD 30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necrosis Factor Rece

Cat:

SL2495R

Species Reactivity：

Human,Pig,(predicted: Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD30:131-230/595<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=0.2μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD30) Polyclonal Antibody, Unconjugated (SL2495R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD30 antibody(SL2495R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Function:
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

Subunit:
Interacts with TRAF1, TRAF2, TRAF3 and TRAF5.

Subcellular Location:
Isoform Long: Cell membrane; Single-pass type I membrane protein.
Isoform Short: Cytoplasm.

Post-translational modifications:
Phosphorylated on serine and tyrosine residues.

Similarity:
Contains 6 TNFR-Cys repeats.

SWISS:
P28908

Gene ID:
943

Database links:

Entrez Gene: 943 Human

Omim: 153243 Human

SwissProt: P28908 Human

Unigene: 1314 Human

CD30是一种单链糖蛋白，标记活化T和B淋巴细胞，R-S细胞和部分淋巴滤泡间区免疫母细胞。CD30是霍奇金淋巴瘤的一个特异性标记，也是大细胞间变型淋巴瘤的诊断型标志。 Picture

Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD30 antibody(SL2495R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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