Rabbit Anti-ELAVL1 antibody

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HuR / ELAVL1; ELAV (embryonic lethal abnormal vision Drosophila) like 1; ELAV like 1; ELAV-like protein 1; ELAV1; ELAV1_HUMAN; Embryonic lethal abnormal vision drosophila homolog like 1; Hu Antigen R; Hu-antigen R; Hua; HuR; MelG.

Cat:

SL2651R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ELAVL1:251-326/326

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Jurkat(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti-ELAVL1 (SL2651R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDTissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ELAVL1 Polyclonal Antibody, Unconjugated(SL2651R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ELAVL1 Polyclonal Antibody, Unconjugated(SL2651R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression. [provided by RefSeq].

Function:
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro.

Subunit:
Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2.

Subcellular Location:
Cytoplasm. Nucleus. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Phosphorylated by MAPKAPK2.
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.

Similarity:
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains.

SWISS:
Q15717

Gene ID:
1994

Database links:

Entrez Gene: 1994 Human

Entrez Gene: 15568 Mouse

Entrez Gene: 363854 Rat

Omim: 603466 Human

SwissProt: Q15717 Human

SwissProt: P70372 Mouse

Unigene: 184492 Human

Unigene: 713744 Human

Unigene: 119162 Mouse

Picture

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ELAVL1 Polyclonal Antibody, Unconjugated(SL2651R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: human carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ELAVL1 Polyclonal Antibody, Unconjugated(SL2651R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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