Rabbit Anti-IL-22 antibody | Sunlong Medical

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Interleukin-22; Il22; Iltif; ILTIFa; MGC129416; MGC129417; IL-10-related T-cell-derived-inducible factor; IL-TIF; ILTIF; IL-TIF alpha; Interleukin-22a; IL-22a; IL 21; IL D110; IL TIF; IL21; IL22; ILD110; interleukin 21; MGC79382; MGC79384; TIFa; TIFIL 23;

Cat:

SL2623R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human IL-22:35-179/179

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: 293T (Human) Cell Lysate at 30 ugLane 2: A549 (Human) Cell Lysate at 30 ugPrimary: Anti-IL22 (SL2623R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 20 kDObserved band size: 18 kDSample: A549(Human) Cell Lysate at 30 ugPrimary: Anti-IL22 (SL2623R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 19 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (SL2623R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-IL22 antibody(SL2623R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri. [provided by RefSeq].

Function:
Cytokine that contributes to the inflammatory response in vivo.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-10 family.

SWISS:
Q9GZX6

Gene ID:
50616

Database links:

Entrez Gene: 50616 Human

Entrez Gene: 50929 Mouse

Entrez Gene: 500836 Rat

Omim: 605330 Human

SwissProt: Q9GZX6 Human

SwissProt: Q9JJY9 Mouse

Unigene: 287369 Human

Unigene: 103585 Mouse

Unigene: 195879 Rat

IL-22是白介素-10家族的成员,主要是由激活的T 淋巴细胞产生的一类重要的细胞因子,除了在Th17细胞中高表达之外，在NK和NKT等细胞中也有表达,在组织损伤修复以及牛皮癣、肝炎、肺炎、肠炎等慢性炎症中发挥重要作用.IL-22是继IL-6和IL-10之后又一个参与炎症反应和代谢调控的细胞因子，IL-22有可能成为干预脂肪肝发生发展的新方法。 Picture

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-IL22 (SL2623R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (SL2623R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-IL22 antibody(SL2623R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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