Rabbit Anti-phospho-Nrf2 (Ser40) antibody

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Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear factor-E2 related factor2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.

Cat:

SL2013R

Species Reactivity：

Human,Mouse,Rat,Pig,(predicted: Chicken,Dog,Cow,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40:DF(p-S)QR

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: HepG2 (Human) Cell Lysate at 30 ugLane 3: MCF-7 (Human) Cell Lysate at 30 ugLane 4: Jurkat (Human) Cell Lysate at 30 ugLane 5: B16 (Human) Cell Lysate at 30 ugLane 6: A549 (Human) Cell Lysate at 30 ugPrimary: Anti-phospho-Nrf2 (Ser40) (SL2013R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 68 kDObserved band size: 135 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (SL2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (SL2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (SL2013R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (SL2013R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNSLCbZIP) subfamily of basic /leucine zipper (bZIP) transcription factors.

Function:
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Subunit:
Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.

Tissue Specificity:
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Post-translational modifications:
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

Similarity:
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.

SWISS:
Q16236

Gene ID:
4780

Database links:

Entrez Gene: 4780 Human

Omim: 600492 Human

SwissProt: Q16236 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (SL2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (SL2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (SL2013R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (SL2013R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

Product Feedback Wall

Specific References (38) | SL2013R has been referenced in 38 publications.

[IF=3.37] Kakehashi A, Kato A, Ishii N, Wei M, Morimura K, et al. (2014) Valerian Inhibits Rat Hepatocarcinogenesis by Activating GABA(A) Receptor-Mediated Signaling. PLoS ONE 9(11): e113610. IHSLCP ; Rat.

PubMed:25419570

[IF=3.71] Ko, Su Hyuk, et al. "Crude Preparations of Helicobacter pylori Outer Membrane Vesicles Induce Upregulation of Heme Oxygenase-1 via Activating Akt-Nrf2 and mTOR-IκB Kinase-NF-κB Pathways in Dendritic Cells." Infection and Immunity (2016): IAI-00190. IF(ICC) ; Others.

PubMed:27185786

[IF=9.274] Wiebke Sachs. et al. Distinct Modes of Balancing Glomerular Cell Proteostasis in Mucolipidosis Type II and III Prevent Proteinuria. J Am Soc Nephrol. 2020 Aug;31(8):1796-1814 WB,IF ; Mouse.

PubMed:32641396

[IF=6.922] Yunxiao Zhong. et al. Nrf2 Inhibits the Progression of Parkinson’s Disease by Upregulating AABR07032261.5 to Repress Pyroptosis. J Inflamm Res. 2022 Feb;15:669-685 WB ; Rat.

PubMed:35140498

[IF=4.119] Cheng-You Lu. et al. Calycosin alleviates H2O2-induced astrocyte injury by restricting oxidative stress through the Akt/Nrf2/HO-1 signaling pathway. 2022 Jan 06 WB ; Rat.

PubMed:34990515

[IF=5.187] Ana L. Vivinetto. et al. Myelin-associated glycoprotein activation triggers glutamate uptake by oligodendrocytes in vitro and contributes to ameliorate glutamate-mediated toxicity in vivo. Bba-Mol Basis Dis. 2022 Apr;1868:166324 IF ; Rat.

PubMed:34954343

[IF=4.315] Hao Huang. et al. Maintaining blood retinal barrier homeostasis to attenuate retinal ischemia-reperfusion injury by targeting the KEAP1/NRF2/ARE pathway with lycopene. Cell Signal. 2021 Dec;88:110153 WB,IF ; Human.

PubMed:10.1016/j.cellsig.2021.110153

[IF=7.376] Ziling Wang. et al. Ginsenoside Rg1 prevents bone marrow mesenchymal stem cell senescence via NRF2 and PI3K/Akt signaling. Free Radical Bio Med. 2021 Aug;: WB ; Mouse.

PubMed:34364981

[IF=7.376] Ziling Wang. et al. Ginsenoside Rg1 prevents bone marrow mesenchymal stem cell senescence via NRF2 and PI3K/Akt signaling. Free Radical Bio Med. 2021 Aug;: WB ; Mouse.

PubMed:34364981

[IF=3.411] Ko SH et al. Bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in dendritic cells via reactive oxygen species-, mitogen-activated protein kinase-, and Nrf2-dependent pathway. World J Gastroenterol. 2020 Jan 21;26(3):291-306. WB&ICF ; Mouse.

PubMed:31988590

[IF=2.148] Gęgotek A et al. Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation. Arch Dermatol Res. 2019 Apr;311(3):203-219. WB ; Human.

PubMed:30783768

[IF=3.961] Ghosh et al. Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice. (2017) Endocrinology. 158:903-919 WB ; Mouse.

PubMed:2834805

[IF=3.73] Ko, Su Hyuk, et al. "Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinases-and NF-κB-Dependent Pathway, Leading to Modulation of Apoptosis." Infection and Immunity (2016): IAI-00191. IF(ICC) ; Mouse.

PubMed:27324483

[IF=5.03] Yen, Ting-Lin, et al. "Andrographolide stimulates p38 MAPK-Nrf2-HO-1 signaling in primary cerebral endothelial cells for definite protection against ischemic stroke in rats." Translational Research (2016). WB ; Mouse.

PubMed:26741362

[IF=3.03] Allard, Joanne S., et al. "Prolonged metformin treatment leads to reduced transcription of Nrf2 and neurotrophic factors without cognitive impairment in older C57BL/6J mice." Behavioural Brain Research (2016). WB ; Mouse.

PubMed:26691680

[IF=2.1] Chang, Wen-Chang, et al. "Scopoletin Protects against Methylglyoxal-Induced Hyperglycemia and Insulin Resistance Mediated by Suppression of Advanced Glycation Endproducts (AGEs) Generation and Anti-Glycation." Molecules 20.2 (2015): 2786-2801. IHSLCP ; Rat.

PubMed:25671364

[IF=4.48] Cárdeno, Ana, et al. "Squalene targets pro-and anti-inflammatory mediators and pathways to modulate over-activation of neutrophils, monocytes and macrophages." Journal of Functional Foods 14 (2015): 779-790. WB ; Human.

PubMed:doi:10.1016/j.jff.2015.03.009

[IF=7.19] Mo, Chunfen, et al. "The Crosstalk between Nrf2 and AMPK Pathways Is Important for the Anti-inflammatory Effect of Berberine in LPS-Stimulated Macrophages and Endotoxin-Shocked Mice."?Antioxidants & redox signaling?ja (2013).

PubMed:23875776

[IF=7.446] Jianbing Wu. et al. Design, Synthesis, and Biological Evaluation of Organic Nitrite (NO2–) Donors as Potential Anticerebral Ischemia Agents. J Med Chem. 2021;XXXX(XXX):XXX-XXX IHC ; Rat.

PubMed:34292749

[IF=8.071] Ming Qi. et al. Ellagic acid ameliorates paraquat-induced liver injury associated with improved gut microbial profile. Environ Pollut. 2022 Jan;293:118572 WB ; Pig.

PubMed:34838710

[IF=6.205] Michelle L. Kerns. et al. Pathogenic and therapeutic role for NRF2 signaling in ultraviolet light–induced skin pigmentation. Jci Insight. 2020 Oct 15; 5(20): e139342 IF,IHC ; Human, Mouse.

PubMed:33001866

[IF=2.05] Ibtissem Rahim. et al. Melatonin alleviates sepsis-induced heart injury through activating the Nrf2 pathway and inhibiting the NLRP3 inflammasome. N-S Arch Pharmacol. 2021 Feb;394(2):261-277 WB ; Mouse.

PubMed:32936353

[IF=3.531] Szilamér Ferenczi. et al. Effects of Single and Repeated Oral Doses of Ochratoxin A on the Lipid Peroxidation and Antioxidant Defense Systems in Mouse Kidneys. Toxins. 2020 Nov;12(11):732 WB ; Mouse.

PubMed:33266415

[IF=5.201] Marina K et al. Mesenchymal Stem Cells Early Response to Low-Dose Ionizing RadiationFront Cell Dev Biol.2020 Dec 14;8:584497. FCM ; Human.

PubMed:33381502

[IF=3.36] Li, Ying-Na, et al. "Saponins from Aralia taibaiensis Attenuate D-Galactose-Induced Aging in Rats by Activating FOXO3a and Nrf2 Pathways." Oxidative medicine and cellular longevity 2014 (2014). IHSLCP ;

PubMed:24669284

[IF=4.556] Jong Ik Jeonet al. Bacteroides fragilis Enterotoxin Induces Sulfiredoxin-1 Expression in Intestinal Epithelial Cell Lines Through a Mitogen-Activated Protein Kinases- and Nrf2-Dependent Pathway, Leading to the Suppression of Apoptosis. Int J Mol Sci . 2020 Jul 29;21(15):5383. WB ; Human.

PubMed:32751114

[IF=4.716] Gong H et al. Nrf2-SHP Cascade-Mediated STAT3 Inactivation Contributes to AMPK-Driven Protection Against Endotoxic Inflammation. Front Immunol. 2020 Mar 10;11:414. WB ; Mouse.

PubMed:32210977

[IF=3.55] Cheng et al. Resveratrol protects against methylglyoxal-induced hyperglycemia and pancreatic damage in vivo. (2015) Nutrient. 7:2850-65 IHSLCF ; Mouse.

PubMed:25884658

[IF=4.29] Ghosh, Anindya, et al. "Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice." Endocrinology 158.4 (2017): 903-919. WB ; Mouse.

PubMed:2834805

[IF=2.91] Hsu, Wei-Hsuan, et al. "Monascin and AITC attenuate methylglyoxal-induced PPAR?? phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation."Journal of agricultural and food chemistry (2013).. Mouse.

PubMed:23731245

[IF=2.74] Cheng, An-Sheng, Yu-Hsiang Cheng, and Tsu-Liang Chang. "Resveratrol protects RINm5F pancreatic cells from methylglyoxal-induced apoptosis." Journal of Functional Foods (2013). WB ; Rat.

PubMed:10.1016/j.jff.2013.07.021

[IF=3.14] Hou, Xiang, et al. "Ameliorative effect of ampelopsin on LPS-induced acute phase response in piglets." Journal of Functional Foods 35 (2017): 489-498. WB ; Pig.

PubMed:10.1016/j.jff.2017.05.044

[IF=3.85] Kim, Mi-Hwi, et al. "EX4 stabilizes and activates Nrf2 via PKCδ, contributing to the prevention of oxidative stress-induced pancreatic beta cell damage." Toxicology and Applied Pharmacology (2016). WB ; Rat.

PubMed:27939242

[IF=5.74] Santofimia-Castaño, Patricia, et al. "Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca 2+ influx activation in mouse pancreatic acinar cells." Free Radical Biology and Medicine (2015). Rat.

PubMed:26163001

[IF=2.87] Kaneko, Yoko S., et al. "Aripiprazole increases NAD (P) H–quinone oxidoreductase-1 and heme oxygenase-1 in PC12 cells." Journal of Neural Transmission: 1-16. WB ; Rat.

PubMed:25504008

[IF=3.01] Lee, Bao-Hong, et al. "< i> Graptopetalum paraguayense and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." Food and Chemical Toxicology 62 (2013): 492-498. WB ; Mouse.

PubMed:4836142

[IF=5.27] Lee, Bao-Hong, et al. "Ankaflavin: A natural novel PPAR?? agonist up-regulated Nrf2 to attenuate methylglyoxal (MG)-induced diabetes< i> in vivo."Free Radical Biology and Medicine (2012).y WB ; Rat.

PubMed:2302488

[IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine WB ; Human, Mouse.

PubMed:23434766