Rabbit Anti-LC3B antibody | Sunlong Medical

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LC3II; LC-3Ⅱ; ATG8F; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3 like protein 2; MAP1 light chain 3-lik

Cat:

SL2912R

Species Reactivity：

Human,Mouse,Rat,Rabbit,(predicted: Chicken,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human LC3A/B:21-121/121

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (SL2912R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (SL2912R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (SL2912R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.

Function:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).

Subunit:
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.

Subcellular Location:
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes.

Tissue Specificity:
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Post-translational modifications:
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Similarity:
Belongs to the MAP1 LC3 family.

SWISS:
Q9GZQ8

Gene ID:
81631

Database links:

Entrez Gene: 427559 Chicken

Entrez Gene: 408001 Cow

Entrez Gene: 81631 Human

Entrez Gene: 67443 Mouse

Entrez Gene: 64862 Rat

Omim: 609604 Human

SwissProt: O41515 Cow

SwissProt: Q9GZQ8 Human

SwissProt: Q9CQV6 Mouse

SwissProt: Q62625 Rat

Unigene: 356061 Human

Unigene: 28357 Mouse

Unigene: 41412 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (SL2912R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (SL2912R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Product Feedback Wall

Specific References (13) | SL2912R has been referenced in 13 publications.

[IF=13.281] Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 20 IF ; Mouse.

PubMed:34672076

[IF=3.647] Jihang Dai. et al. Glabridin inhibits osteoarthritis development by protecting chondrocytes against oxidative stress, apoptosis and promoting mTOR mediated autophagy. Life Sci. 2021 Mar;268:118992 IHC ; Rat.

PubMed:33417956

[IF=2.107] Yin X et al. Lithium facilitates removal of misfolded proteins and attenuated faulty interaction between mutant SOD1 and p-CREB (Ser133) through enhanced autophagy in mutant hSOD1G93A transfected neuronal cell lines. Mol Biol Rep. 2019 Sep 16. WB ; Human.

PubMed:31529340

[IF=2.589] Wang WJ et al. Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis. Reprod Biol Endocrinol. 2019 Jan 3;17(1):2. IHSLCP,IHF&WB ; Human.

PubMed:30606202

[IF=2.1] Ruiqiao Guan. et al. Electroacupuncture at GV20‑GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis. Mol Med Rep. 2021 Jul;24(1):1-15 IF ; Rat.

PubMed:33955500

[IF=7.727] Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306 IHC ; Mouse.

PubMed:34081995

[IF=1.591] Zihua Liu. et al. Overexpression of thioredoxin reductase 1 can reduce DNA damage, mitochondrial autophagy and endoplasmic reticulum stress in Parkinson's disease. 2020 Nov 23 WB,IF,IHC ; Mouse.

PubMed:33230666

[IF=6.022] Liwen Luo. et al. Cartilage endplate stem cells inhibit intervertebral disc degeneration by releasing exosomes to nucleus pulposus cells to activate Akt/autophagy. Stem Cells. 2021 Jan 18 IF ; Rat.

PubMed:33459443

[IF=5.026] Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKIIα-associated activation of autophagy. Cell Biosci . 2020 Jul 30;10:90. IHC ; Human.

PubMed:32760495

[IF=3.647] Lin D et al. Aldehyde dehydrogenase 2 regulates autophagy via the Akt-mTOR pathway to mitigate renal ischemia-reperfusion injury in hypothermic machine perfusion. Life Sci . 2020 Jul 15;253:117705. IHC,WB ; mouse.

PubMed:32334008

[IF=1.445] Li YH et al. Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/β-Catenin Signaling. Chin J Integr Med. 2020 Jan 4. WB ; Mouse&Rat.

PubMed:31903532

[IF=0] Zhang P et al. Human metapneumovirus induces autophagy through JNK and MEK/ERK pathway. assets.researchsquare.2019. ICF ; Human.

PubMed:doi:10.21203/rs.2.17332/v1

[IF=2.173] Liu B et al. Effects of Autophagy on Synaptic-Plasticity-Related Protein Expression in the Hippocampus CA1 of a Rat Model of Vascular Dementia. Neurosci Lett. 2019 Jun 1;707:134312. WB ; Rat.

PubMed:31163225