Rabbit Anti-TBX18 antibody | Sunlong Medical

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T box 18; T box protein 18; T box transcription factor TBX18; T-box protein 18; T-box transcription factor TBX18; TBX18; TBX18_HUMAN.

Cat:

SL4748R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human TBX18:201-300/607

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HT29 Cell lysate at 30ug; Primary: Anti-TBX18 (SL4748R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size: 65 kDObserved band size: 65 kDSample：line1：Mouse embryo lysate at 45ug; line2：Mouse brain lysate at 45ug; Primary: Anti-TBX18(SL4748R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 65kDObserved band size : 67kDWe are unsure as to the identity of these extra bandsParaformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBX18) Polyclonal Antibody, Unconjugated (SL4748R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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T-box transcription factors are a group of phylogenetically conserved genes that contain a uniquely defining DNA-binding domain, the T-box domain. These genes are believed to be involved in the regulation of development processes, for example the development of limbs, and it is known that haploinsufficiency of multiple T-box proteins results in severe human congenital malformation syndromes, involving craniofacial, cardiovascular, and skeletal structures. TBX 18 has been reported to be involved in numerous development processes and to act as an antiapoptotic factor.

Function:
Probable transcriptional regulator involved in developmental processes.

Subunit:
Homodimer. Can form a heterodimer with TBX15. Interacts with GATA4 AND NKX2-5. Interacts with PAX3 (By similarity). Interacts (via engrailed homology 1 repressor motif) with TLE3; this interaction represses TBX18 transcriptional activity (By similarity) (PubMed:26235987). Interacts with SIX1 (PubMed:26235987).

Subcellular Location:
Nucleus.

DISEASE:
Congenital anomalies of kidney and urinary tract 2 (CAKUT2). The disease is caused by mutations affecting the gene represented in this entry. A disorder encompassing a broad spectrum of renal and urinary tract malformations that include renal agenesis, kidney hypodysplasia, multicystic kidney dysplasia, duplex collecting system, posterior urethral valves and ureter abnormalities. Congenital anomalies of kidney and urinary tract are the commonest cause of chronic kidney disease in children.

Similarity:
Contains 1 T-box DNA-binding domain.

SWISS:
O95935

Gene ID:
9096

Database links:

Entrez Gene: 9096 Human

Entrez Gene: 76365 Mouse

Entrez Gene: 315870 Rat

Omim: 604613 Human

SwissProt: O95935 Human

SwissProt: Q9EPZ6 Mouse

Unigene: 251830 Human

Unigene: 158789 Mouse

Unigene: 161921 Rat

Picture

Sample：
line1：Mouse embryo lysate at 45ug;
line2：Mouse brain lysate at 45ug;
Primary: Anti-TBX18(SL4748R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 65kD
Observed band size : 67kD
We are unsure as to the identity of these extra bands

Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBX18) Polyclonal Antibody, Unconjugated (SL4748R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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