Rabbit Anti-CYP11A1 antibody

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Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase (side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HU

Cat:

SL728R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC:321-420/521

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Placenta (Mouse) Lysate at 30 ugPrimary: Anti- CYP11A1 (Ser576) (SL728R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 53/57 kDObserved band size: 57 kDSample: Lane1: Brain(Rat) Lysate at 30 ugLane2: Kidney(Rat)Lysate at 30 ugPrimary: Anti-CYP11A1/P450SCC (SL728R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 53kDObserved band size : 53kDParaformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB stainingParaformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Function:
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Subcellular Location:
Mitochondrion membrane.

DISEASE:
Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
P05108

Gene ID:
1583

Database links:

Entrez Gene: 1583 Human

Omim: 118485 Human

SwissProt: P05108 Human

Unigene: 30796 Human

Picture

Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Kidney(Rat)Lysate at 30 ug
Primary: Anti-CYP11A1/P450SCC (SL728R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 53kD
Observed band size : 53kD

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (SL728R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Specific References (12) | SL728R has been referenced in 12 publications.

[IF=2.74] Jianyu Ma. et al. Melatonin alleviated oxidative stress induced by energy restriction on sheep Leydig cells through Sirt1/Sod2 pathway. Theriogenology. 2021 Jul;: WB ; Sheep.

PubMed:34352672

[IF=4.167] Xiaopeng An. et al. Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1. J Anim Sci Biotechno. 2020 Dec;11(1):1-20 WB ; Goat.

PubMed:33072314

[IF=3.585] Sun J et al. Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di (2-ethylhexyl) phthalate. Toxicol Appl Pharmacol. 2019 Jun 6;378:114612. ICF&WB ; Mouse.

PubMed:31175881

[IF=4.095] Jeremy M et al. Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model.J Steroid Biochem Mol Biol. 2019 Jun;190:64-75. WB ; Rat.

PubMed:30923019

[IF=15.221] Qiu JT et al. Micro RNA‐7 inhibits melatonin synthesis by acting as a linking molecule between leptin and norepinephrine signaling pathways in pig pineal gland. Journal of Pineal Research,2019 e12552. IHSLCP ; Pig.

PubMed:doi:10.1111/jpi.12552

[IF=3.457] Sagaradze GD et al. Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential.(2018)Biomed Pharmacother;109:1428-1436. IHC ; Rat.

PubMed:30551394

[IF=3.86] Ommati, Mohammad Mehdi, et al. "Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models." Toxicology letters (2017). WB ; Rat.

PubMed:29197623

[IF=2.08] Medwid, Samantha, Haiyan Guan, and Kaiping Yang. "Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring."Environmental Toxicology and Pharmacology (2016). WB ; Mouse.

PubMed:27017381

[IF=2.6] Mahata, Bidesh, et al. "Single-Cell RNA Sequencing Reveals T Helper Cells Synthesizing Steroids De Novo to Contribute to Immune Homeostasis." Cell Reports (2014). Mouse.

PubMed:24813893

[IF=2.14] Hu, Yanhui, et al. "Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells." Reproductive Biology and Endocrinology 11.1 (2013): 72. WB ; Mouse.

PubMed:23889939

[IF=2.823] Lalrawngbawli et al. Effect of metformin on testicular expression and localization of leptin receptor and levels of leptin in the diabetic mice. Mol Reprod Dev . 2020 May;87(5):620-629. WB ; mouse.

PubMed:32249489

[IF=4.067] Wang X et al. Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells. Int J Biol Sci. 2019 Jan 1;15(2):404-415. WB ; Cow.

PubMed:30745830