Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Spleen (Mouse) Lysate at 40 ug
Lane 6: Lymph node (Mouse) Lysate at 40 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 9: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Vimentin (Ser56) (SL3471R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti- Phospho-Vimentin (Ser56) antibody (SL3471R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.