Rabbit Anti-DIO2 antibody | Sunlong Medical

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5DII; D2; Deiodinase, iodothyronine, type II; DIOII; ITDI2; SelY; Thyroxine deiodinase, type II; TXDI2; Type 2 DI; Type II 5' deiodinase; Type II iodothyronine deiodinase.

Cat:

SL3673R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human DIO2:101-200/273

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-DIO2 (SL3673R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 30 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (SL3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (SL3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.

Function:
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

Subunit:
Interacts with USP20 and USP33. Interacts with MARCH6.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential).

Tissue Specificity:
Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain.

Post-translational modifications:
Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.

Similarity:
Belongs to the iodothyronine deiodinase family.

SWISS:
Q92813

Gene ID:
1734

Database links:

Entrez Gene: 494548 Cow

Entrez Gene: 1734 Human

Entrez Gene: 13371 Mouse

Entrez Gene: 414379 Pig

Entrez Gene: 65162 Rat

Omim: 601413 Human

SwissProt: Q5I3B2 Cow

SwissProt: Q92813 Human

SwissProt: Q9Z1Y9 Mouse

SwissProt: Q6QN12 Pig

SwissProt: P70551 Rat

Unigene: 202354 Human

Unigene: 21389 Mouse

Unigene: 408815 Mouse

Unigene: 88380 Rat

脱碘酶2泛称Ⅱ型碘甲腺氨酸脱碘酶脱碘，是调节甲状腺激素生物活性的特别方式。脱碘酶可加速T4转化为T3，引起甲状腺激素及生长激素分泌减少，影响一些机体生物学的改变。 DIO2主要表达在中枢神经系统、垂体前叶腺体、棕色脂肪组织和胎盘内。其他组织中也有一定量的分布：心肌和骨胳肌、肝、肾、中枢神经系统、脑，皮肤，胎盘等。 Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (SL3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (SL3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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