The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.
Subunit:
NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1.
Subcellular Location:
Cell projection, invadopodium membrane; Multi-pass membrane protein.
Tissue Specificity:
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
Similarity:
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
SWISS:
Q9Y5S8
Gene ID:
27035
Database links:
Entrez Gene: 27035 Human
Entrez Gene: 237038 Mouse
Omim: 300225 Human
SwissProt: Q9Y5S8 Human
SwissProt: Q8CIZ9 Mouse
Unigene: 592227 Human
Unigene: 233865 Mouse
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Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-Nox1 (SL3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- Nox1 (SL3682R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti- Nox1 (SL3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD
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