Rabbit Anti-DGAT1 antibody | Sunlong Medical

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ACAT related gene product 1; ACAT-related gene product 1; Acyl coenzyme A:cholesterol acyltransferase related gene 1; Acyl-CoA retinol O-fatty-acyltransferase; Acyl-CoA:diacylglycerol acyltransferase; ARAT; ARGP1; C75990; D15Ertd23e; Dgat; DGAT1

Cat:

SL2332R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human DGAT1:251-350/488

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela (human)cell Lysate at 40 ugPrimary: Anti- DGAT1 (SL2332R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55kDObserved band size: 55 kDSample: Lane 1: Mouse Adipose tissue lysates Lane 2: Mouse Adrenal gland tissue lysates Lane 3: Rat Adipose tissue lysates Lane 4: Rat Stomach tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Human HL60 cell lysates Lane 7: Human HepG2 cell lysates Primary: Anti-DGAT1 (SL2332R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 60 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, Unconjugated (SL2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Function:
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Subunit:
Homodimer or homotetramer.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein.

Similarity:
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

SWISS:
O75907

Gene ID:
8694

Database links:

Entrez Gene: 482093 Dog

Entrez Gene: 8694 Human

Entrez Gene: 13350 Mouse

Entrez Gene: 84497 Rat

Omim: 60980 Human

SwissProt: O75907 Human

SwissProt: Q9Z2A7 Mouse

SwissProt: Q9ERM3 Rat

Unigene: 521954 Human

Unigene: 22633 Mouse

Unigene: 252 Rat

Picture

Sample:
Lane 1: Mouse Adipose tissue lysates
Lane 2: Mouse Adrenal gland tissue lysates
Lane 3: Rat Adipose tissue lysates
Lane 4: Rat Stomach tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Human HL60 cell lysates
Lane 7: Human HepG2 cell lysates
Primary: Anti-DGAT1 (SL2332R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, Unconjugated (SL2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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