Rabbit Anti-NOX2 antibody | Sunlong Medical

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AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytochrome b 245, beta polypeptide; Cytochrome b(558) beta chain; Cytochrome b(558) subunit beta; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP91PHOX;

Cat:

SL3889R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human NOX2:501-570/570<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-NOX2 (SL3889R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65 kDSample: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti-NOX2 (SL3889R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65 kDSample: Lane 1: Rat Bronchus tissue lysates Lane 2: Rat Esophagus tissue lysatesLane 3: Human HL60 cell lysates Primary: Anti-NOX2 (SL3889R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 65 kDaObserved band size: 65 kDaTissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated(SL3889R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.

Function:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

Subcellular Location:
Membrane.

Post-translational modifications:
Glycosylated.

DISEASE:
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:301280]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

Similarity:
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.

SWISS:
P04839

Gene ID:
1536

Database links:

Entrez Gene: 281112 Cow

Entrez Gene: 1536 Human

Entrez Gene: 13058 Mouse

Entrez Gene: 100001761 Rabbit

Entrez Gene: 66021 Rat

Omim: 300481 Human

SwissProt: O46522 Cow

SwissProt: P04839 Human

SwissProt: Q61093 Mouse

Unigene: 292356 Human

Unigene: 200362 Mouse

NOX2也是一个很重要的产生氧自由基的酶。主要存在于甲状腺,前列腺，唾液腺和直肠粘膜中。 Picture

Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-NOX2 (SL3889R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD

Sample:
Lane 1: Rat Bronchus tissue lysates
Lane 2: Rat Esophagus tissue lysates
Lane 3: Human HL60 cell lysates
Primary: Anti-NOX2 (SL3889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated(SL3889R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Specific References (25) | SL3889R has been referenced in 25 publications.

[IF=5.33] Shuji Wakatsuki. et al. Selective phosphorylation of serine 345 on p47-phox serves as a priming signal of ROS-mediated axonal degeneration. Exp Neurol. 2022 Jun;352:114024 WB ; Mouse.

PubMed:10.1016/j.expneurol.2022.114024

[IF=6.312] Lei Chen. et al. Therapeutic Potential of Perillaldehyde in Ameliorating Vulvovaginal Candidiasis by Reducing Vaginal Oxidative Stress and Apoptosis. Antioxidants-Basel. 2022 Feb;11(2):178 WB ; Mouse.

PubMed:10.3390/antiox11020178

[IF=8.401] Mr. Aleksandr Vendrov. et al. Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. 2021 Oct 29 IF,IHC ; Mouse.

PubMed:34714114

[IF=6.633] Yun Li. et al. The voltage-gated proton channel Hv1 plays a detrimental role in contusion spinal cord injury via extracellular acidosis-mediated neuroinflammation. Brain Behav Immun. 2021 Jan;91:267 FC ; Mouse.

PubMed:33039662

[IF=9.038] Xie C et al. Mechanism investigation on Bisphenol S-induced oxidative stress and inflammation in murine RAW264.7 cells: The role of NLRP3 inflammasome, TLR4, Nrf2 and MAPK. J Hazard Mater . 2020 Jul 15;394:122549. WB，IF ; mouse.

PubMed:32283380

[IF=0] Liu HB et al. Celastrol alleviates aortic valve calcification via inhibition of NADPH oxidase-2 in valvular interstitial cells. JACC: Basic to Translational Science. 18 December 2019. WB&IHC&ICF ; Human.

PubMed:doi:10.1016/j.jacbts.2019.10.004

[IF=1.84] Köroğlu KM et al. Apocynin alleviates cisplatin‐induced testicular cytotoxicity by regulating oxidative stress and apoptosis in rats. Andrologia. 2019 May;51(4):e13227. IHSLCP ; Rat.

PubMed:30623469

[IF=0] Hu, Xiaoqin, et al. "Effects of Resveratrol on Oxidative Stress Injury Induced by Rapid-Pacing in Isolated Rabbit Hearts." Journal of Developing Drugs 3.128 (2014): 2. WB ; Rabbit.

PubMed:doi:10.15369/sujms.28.89

[IF=22.634] Lagadinou, Eleni D., et al. "BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells." Cell stem cell 12.3 (2013): 329-341. FCM ; Human.

PubMed:23333149

[IF=1.25] Kashiwabara, Yoshiaki, et al. "Immunohistochemical localization of Nox in mouse circumvallate papillae." Tissue and Cell (2015). IHSLCP ; Mouse.

PubMed:26603975

[IF=9.83] Wakatsuki, Shuji, et al. "Oxidative stress–dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration." The Journal of Cell Biology (2015): jcb-201506102. IHSLCP ; Mouse.

PubMed:26572622

[IF=7.19] Wang, Yabin, et al. "Activation of Liver X receptor improves viability of adipose-derived mesenchymal stem cells (AD-MSCs) to attenuate myocardial ischemia injury through TLR4/NF-κB and Keap-1/Nrf2 signaling pathways." Antioxidants & Redox Signaling ja (2014). WB ; Mouse.

PubMed:24915051

[IF=5.778] WB ; Rat.

PubMed:33017703

[IF=3.21] Dalaklioglu, Selvinaz, et al. "Metastatic breast carcinoma induces vascular endothelial dysfunction in Balb-c mice: Role of the tumor necrosis factor-?? and NADPH oxidase."Vascular Pharmacology (2013).& Mouse.

PubMed:23933568

[IF=5.81] Xiaoyu Liu. et al. Geniposide Combined With Notoginsenoside R1 Attenuates Inflammation and Apoptosis in Atherosclerosis via the AMPK/mTOR/Nrf2 Signaling Pathway. Front Pharmacol. 2021; 12: 687394 WB ; Mouse, Human.

PubMed:34305600

[IF=6.792] Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345 WB ; Human.

PubMed:34004477

[IF=5.984] Rodney M. Ritzel. et al. Proton extrusion during oxidative burst in microglia exacerbates pathological acidosis following traumatic brain injury. Glia. 2021 Mar;69(3):746-764 FC ; Mouse.

PubMed:33090575

[IF=5.778] Zixuan Liu. et al. Tetrachlorobenzoquinone exposure triggers ferroptosis contributing to its neurotoxicity. Chemosphere. 2021 Feb;264:128413 WB ; Rat.

PubMed:33017703

[IF=2.73] Cheng Yu. et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring. Microvasc Res. 2021 Jan;133:104076 WB ; Rat.

PubMed:32956647

[IF=4.96] Xue-Qi Liuet al. Rutaecarpine Derivative Cpd-6c Alleviates Acute Kidney Injury by Targeting PDE4B, a Key Enzyme Mediating inflammation in Cisplatin Nephropathy. Biochem Pharmacol . 2020 Oct;36:114132. WB ;

PubMed:32622666

[IF=4.171] Zhao Y et al. Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS. Nutrients. 2020 Feb 27;12(3). pii: E616. WB ; Mouse.

PubMed:32120800

[IF=3.448] Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26. WB ; mouse.

PubMed:32100911

[IF=1.851] Yuan X et al. Aldosterone promotes renal interstitial fibrosis via the AIF‑1/AKT/mTOR signaling pathway. Mol Med Rep. 2019 Sep 13. WB ; Rat&Mouse.

PubMed:31545432

[IF=4.868] Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823. WB ; broiler.

PubMed:31467636

[IF=3] Ye, Zhen-Nan, et al. "Inhibition of leukotriene B4 synthesis protects against early brain injury possibly via reducing the neutrophil-generated inflammatory response and oxidative stress after subarachnoid hemorrhage in rats." Behavioural Brain Research (2017). IHSLCP ; Rat.

PubMed:29133197