Home > Product > Antibody > Rabbit Anti-ACOX2 antibody
ACOX 2; Acyl Coenzyme A oxidase 2; Acyl Coenzyme A oxidase 2 branched chain; Acyl coenzyme A oxidase 2 peroxisomal; BCOX; BRCACOX; BRCOX; Peroxisomal branched chain acyl CoA oxidase; THCA CoA oxidase; THCCox; Trihydroxycoprostanoyl CoA oxidase; 3 alpha 7
Cat:
SL5030R
Species Reactivity:
Mouse,Rat,(predicted: Human,Cow,)
Immunogen:
KLH conjugated synthetic peptide derived from human ACOX2:341-440/681
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


The product of this gene belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe mental retardation, and death in children. [provided by RefSeq, Mar 2009]

Function:
Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids.

Subunit:
Heterodimer

Subcellular Location:
Peroxisome.

Tissue Specificity:
Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.

Similarity:
Belongs to the acyl-CoA oxidase family

SWISS:
Q99424

Gene ID:
8309

Database links:

Entrez Gene: 8309 Human

Entrez Gene: 514969 Cow

Entrez Gene: 93732 Mouse

Entrez Gene: 252898 Rat

Omim: 601641 Human

SwissProt: Q99424 Human

SwissProt: Q9QXD1 Mouse

SwissProt: O02767 Rabbit

SwissProt: P97562 Rat

Unigene: 444959 Human

Unigene: 28700 Mouse

Unigene: 10622 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACOX2) Polyclonal Antibody, Unconjugated (SL5030R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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