Rabbit Anti-IGFBP6 antibody

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IBP 6; IBP-6; IBP6; IBP6_HUMAN; IGF binding protein 6; IGF-binding protein 6; IGFBP 6; IGFBP-6; Insulin like growth factor binding protein 6; Insulin-like growth factor-binding protein 6.

Cat:

SL4064R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human IGFBP6:141-240/240

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: muscle (Mouse) Lysate at 40 ugPrimary: Anti- IGFBP6 (SL4064R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 36 kDSample: Brain (Rat) Lysate at 40 ugIntestine (Mouse) Lysate at 40 ugPrimary: Anti-IGFBP6 (SL4064R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 23 kDObserved band size: 23 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated(SL4064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Function:
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Subcellular Location:
Secreted.

Post-translational modifications:
O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

Similarity:
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.

SWISS:
P24592

Gene ID:
3489

Database links:

Entrez Gene: 3489 Human

Entrez Gene: 16012 Mouse

Entrez Gene: 25641 Rat

Omim: 146735 Human

SwissProt: P24592 Human

SwissProt: P47176 Mouse

SwissProt: P35572 Rat

Unigene: 274313 Human

Unigene: 358609 Mouse

Unigene: 6431 Rat

Picture

Sample:
Brain (Rat) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IGFBP6 (SL4064R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated(SL4064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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