Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Frizzled family members, 7 putative transmembrane domains, and an intracellular SLCterminal tail with a PDZ domain-binding motif. Frizzled 7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. Frizzled 7 expression has been reported in brain, gastrointestinal tract, heart, fetal kidney, fetal lung, placenta, skeletal muscle, and various cancers. ESTs have been isolated from a wide variety of normal and cancer libraries.
Function:
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Subunit:
Interacts with MAGI3 and DVL1.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
Similarity:
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
SWISS:
O75084
Gene ID:
8324
Database links:
Entrez Gene: 8324 Human
Entrez Gene: 374060 Chicken
Entrez Gene: 524255 Cow
Entrez Gene: 488478 Dog
Entrez Gene: 14369 Mouse
Entrez Gene: 30188 Rat
GenBank: NP_003498.1 Human
Omim: 603410 Human
SwissProt: O57329 Chicken
SwissProt: O75084 Human
SwissProt: Q61090 Mouse
Unigene: 173859 Human
Unigene: 297906 Mouse
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Sample:
HepG2(Human) Cell Lysate at 30 ug
Muscle (Mouse) Lysate at 40 ug
Muscle (Rat) Lysate at 40 ug
Primary: Anti-Frizzled 7 (SL5125R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Frizzled 7 (SL5125R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (SL5125R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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