Rabbit Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) antibody

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Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC 2.7.10.1; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor

Cat:

SL5181R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human AXL around the phosphorylation site of Tyr698+Tyr702+Tyr703:KI(p-Y)NGD(p-Y)(p-Y)RQ<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ugLane 2: Hela (Human) Cell Lysate at 30 ugLane 3: 293T (Human) Cell Lysate at 30 ugPrimary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (SL5181R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 140’120 kDObserved band size: 120 kDSample: SW96 Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (SL5181R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 95 kDObserved band size: 120 kDSample:MCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (SL5181R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 95 kDObserved band size: 120 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-AXL(Tyr698+Tyr702+Tyr703) Polyclonal Antibody, Unconjugated (SL5181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma.The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).

Function:
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol-3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.

Subunit:
Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TENC1. Part a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Post-translational modifications:
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

DISEASE:
Note=AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thiroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloptoliferative disorders, prostatic carcinoma cells, or breast cancer.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.

SWISS:
P30530

Gene ID:
558

Database links:

Entrez Gene: 558 Human

Omim: 109135 Human

SwissProt: P30530 Human

Unigene: 590970 Human

Picture

Sample: SW96 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (SL5181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 120 kD

Sample:MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (SL5181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 120 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-AXL(Tyr698+Tyr702+Tyr703) Polyclonal Antibody, Unconjugated (SL5181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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