Rabbit Anti-phospho-HOMER3 (Thr36) antibody

Home > Product > Antibody > Rabbit Anti-phospho-HOMER3 (Thr36) antibody

HOMER3 (phospho T36); p-HOMER3 (phospho T36); HOMER3 (phospho T36); HOMER3 (phospho Thr36); HOMER 3; Homer 3D protein; Homer homolog 3 (Drosophila); Homer neuronal immediate early gene 3; Homer protein homolog 3; Homer3; VESL3; HOME3_HUMAN.

Cat:

SL5392R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36:AL(p-T)VS

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549 Cell (Human) Lysate at 40 ugPrimary: Anti- phospho-HOMER3 (Thr36) (SL5392R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 40 kDObserved band size: 40 kDSample: U937(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-HOMER3 (Thr36) (SL5392R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 40 kDParaformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOMER3 (Thr36)) Polyclonal Antibody, Unconjugated (SL5392R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-termina coiled-coil domain and two leucine zipper motifs that are involved l in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.

Function:
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.

Subunit:
Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.

Subcellular Location:
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=Postsynaptic density of neuronal cells.

Similarity:
Belongs to the Homer family.
Contains 1 WH1 domain.

SWISS:
Q9NSC5

Gene ID:
9454

Database links:

Entrez Gene: 9454 Human

Entrez Gene: 26558 Mouse

Omim: 60960 Human

SwissProt: Q9NSC5 Human

SwissProt: Q99JP6 Mouse

Unigene: 720208 Human

Unigene: 88659 Mouse

Picture

Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-HOMER3 (Thr36) (SL5392R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOMER3 (Thr36)) Polyclonal Antibody, Unconjugated (SL5392R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall