Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Adrenal gland Lysates
Lane 3: Rat Brain Lysates
Lane 4: Rat Adrenal gland Lysates
Lane 5: Human DU145 cell Lysates
Lane 6: Human HepG2 cell Lysates
Lane 7: Human A431 cell Lysates
Lane 8: Human HeLa cell Lysates
Primary: Anti-phospho-MEK1 (Thr386) (SL5413R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43kDa
Observed band size: 50kDa
Sample:
placenta (Mouse) Lysate at 40 ug
Primary: Anti-phospho-MEK1 (Thr386) (Bs- 5413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (SL5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MEK1 (Thr386)) Polyclonal Antibody, Unconjugated (SL5413R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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