Rabbit Anti-PAP2B antibody | Sunlong Medical

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Dri42; Lipid phosphate phosphohydrolase 3; LPP3; PAP 2b; PAP2 beta; PAP2b; Vascular endothelial growth factor and type I collagen inducible protein; VCIP.

Cat:

SL4127R

Species Reactivity：

Mouse,(predicted: Human,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human PAP2B:241-311/311

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti-PAP2B (SL4127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 35 kDSample: Embryo (Mouse) Lysate at 40 ugPrimary: Anti-PAP2B (SL4127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAP2B) Polyclonal Antibody, Unconjugated (SL4127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Phosphatidic acid phosphatase type 2b (PPAP2b) is a member of the phosphatidic acid phosphatase (PAP) family that function in de novo synthesis of glycerolipids as well as in receptor activated signal transduction mediated by phospholipase D. PAP2b catalyzes the conversion of phosphatidic acid to diacylglycerol and hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate. It is a homodimer glycoprotein localized at the cell plasma membrane. Expression promotes adhesion, spreading, and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b induced cell-cell interactions regulate specific intracellular signaling pathways that involve p120-catenin, Fak, Akt, and GSK3? protein kinases.

Function:
Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (SLC1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > SLC1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions.

Subunit:
Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability.

Subcellular Location:
Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Ubiquitously expressed. Highly expressed in heart and placenta.

Post-translational modifications:
N-glycosylated. Contains high-mannose oligosaccharides.

Similarity:
Belongs to the PA-phosphatase related phosphoesterase family.

SWISS:
O14495

Gene ID:
8613

Database links:

Entrez Gene: 8613 Human

Entrez Gene: 67916 Mouse

Entrez Gene: 192270 Rat

Omim: 607125 Human

SwissProt: O14495 Human

SwissProt: Q99JY8 Mouse

SwissProt: P97544 Rat

Unigene: 405156 Human

Unigene: 619002 Human

Unigene: 348326 Mouse

Unigene: 12038 Rat

Picture

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-PAP2B (SL4127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAP2B) Polyclonal Antibody, Unconjugated (SL4127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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