DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.
Cat:
SL4128R
Species Reactivity：
Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human MAP4:701-800/1152
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Placenta (Mouse) Lysate at 40 ugPrimary: Anti- MAP4 (SL4128R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 121 kDObserved band size: 121 kDSample: Testis (Mouse) Lysate at 40 ugPrimary: Anti- MAP4 (SL4128R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 121 kDObserved band size: 121 kDSample: DU145(Human) Cell Lysate at 30 ugPrimary: Anti- MAP4 (SL4128R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 121 kDObserved band size: 121 kD
The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the SLCterminus negatively regulates MAP4 activity to promote microtubule assembly.
Subunit: Interacts with SEPT2; this interaction impedes tubulin-binding.
Subcellular Location: Cytoplasm, cytoskeleton.
Post-translational modifications: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
