Rabbit Anti-phospho-MAP3K7IP1 (Thr431) antibody

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MAP3K7IP1(phospho S431); MAP3K7IP1(phospho S429) mo, rat; MAP3K7IP 1; MAP3K7IP1; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; TAB 1; TAK1 binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinas

Cat:

SL5496R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human MAP3K7IP1 around the phosphorylation site of Thr431:EA(p-T)PT

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:U937 Cell (Human) Lysate at 40 ugHela Cell (Human) Lysate at 40 ugSiha Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-MAP3K7IP1 (Thr431)(SL5496R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56kDObserved band size: 65kDSample: 293T Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-MAP3K7IP1 (Thr431)(SL5496R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 56kDObserved band size: 60kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAP3K7IP1 (Thr431)) Polyclonal Antibody, Unconjugated (SL5496R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta and members of the Toll IL 1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N terminus acts as a dominant negative inhibitor of TGF beta, demonstrating how this protein can function as a mediator between TGF beta receptors and TAK1.

Function:
May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.

Subunit:
Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.

Similarity:
Contains 1 PP2SLClike domain.

SWISS:
Q15750

Gene ID:
10454

Database links:

Entrez Gene: 10454 Human

Entrez Gene: 66513 Mouse

Entrez Gene: 315139 Rat

Omim: 602615 Human

SwissProt: Q15750 Human

SwissProt: Q8CF89 Mouse

Unigene: 507681 Human

Unigene: 288245 Mouse

Picture

Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-MAP3K7IP1 (Thr431)(SL5496R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 60kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAP3K7IP1 (Thr431)) Polyclonal Antibody, Unconjugated (SL5496R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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